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Association of polymorphisms in the peroxisome proliferator-activated receptor γ gene and osteoarthritis of the knee
  1. S Cheng1,
  2. H Afif1,
  3. J Martel-Pelletier1,
  4. M Benderdour2,
  5. J-P Pelletier1,
  6. G Hilal1,
  7. P Haraoui3,
  8. J-P Raynauld3,
  9. D Choquette3,
  10. H Fahmi1
  1. 1Osteoarthritis Research Unit, Department of Medicine, Centre Hospitalier de l’Université de Montréal, Montreal, Quebec, Canada
  2. 2Centre de Recherche, Department of Medicine, Sacré-Coeur Hospital, Montreal
  3. 3Department of Medicine, Centre Hospitalier de l’Université de Montréal
  1. Correspondence to:
    H Fahmi
    Osteoarthritis Research Unit, Notre Dame-Hospital, CR-CHUM, 1560 Sherbrooke East, Pavillon DeSève, Y-2628, Montreal, QC H2L 4M1, Canada; h.fahmi{at}umontreal.ca

Abstract

Objectives: To study the association between two common polymorphisms in the peroxisome proliferator-activated receptor γ (PPARγ) gene and susceptibility to, and severity of, osteoarthritis in a French-Canadian population.

Methods: Genomic DNA was obtained from 172 patients with osteoarthritis and 210 ethnically matched healthy controls. Genotyping for the polymorphisms in the PPARγ gene (Pro12Ala and C1431T) was carried out using polymerase chain reaction–restriction fragment length polymorphism. The standard Kellgren–Lawrence grading score and the French version of the Western Ontario and McMaster Universities Osteoarthritis Index were used to assess the radiological and functional severity of the disease. Estimated haplotypes were generated using the expectation maximisation algorithm. Genotype and allele frequencies were analysed using the χ2 test.

Results: Genotype and allele frequencies for either polymorphism in the PPARγ gene did not differ significantly between patients with osteoarthritis and controls. Moreover, no significant differences were observed after stratification of patients according to age at disease onset, radiological or functional severity. Similarly, haplotype analysis of both polymorphisms in the PPARγ gene showed no association of any haplotype with susceptibility to, or severity of, osteoarthritis.

Conclusion: These findings suggest that the examined polymorphisms in the PPARγ gene do not contribute to susceptibility to, or severity of, osteoarthritis in the French-Canadian population.

  • PPAR, peroxisome proliferator-activated receptor
  • WOMAC Index, Western Ontario and McMaster University Osteoarthritis Index

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Footnotes

  • Funding: This study was supported by grants from the Canadian Institutes of Health Research (IMH-63168), the Fonds de Recherche en Santé du Québec (JC2836) and the Fonds de la Recherche du Centre de Recherche du Centre Hospitalier de l’Université de Montréal. SC is supported by a fellowship from the CIHR Training on Mobility and Posture Deficiencies. HF is a research scholar at FRSQ.

  • Competing interests: None declared.

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