Background: Re-expression of the recombination-activating genes (RAG) in peripheral B cells may be relevant in the development of autoreactive antibodies in autoimmune diseases. The presence of antinuclear antibodies (ANA) as a hallmark of oligoarticular juvenile idiopathic arthritis (o-JIA, early-onset type) indicates a breakdown in immunological tolerance.
Aim: To examine the expression of RAG genes in peripheral blood mature B lymphocytes in patients with o-JIA.
Methods: 777 memory B cells from peripheral blood, CD19+ CD27+ CD5+ or CD19+ CD27+ CD5−, isolated from three ANA+ children with o-JIA and three healthy age-matched children, were examined for the expression of RAG1 and RAG2 mRNA. mRNA transcripts of activation-induced cytidine deaminase and immunoglobulin G were searched to further determine their developmental stage.
Results: mRNA was present for any of the two RAG genes in the B cells of children with JIA and controls. However, the predominance of RAG1 or RAG2 was different. A significantly decreased frequency of RAG2-expressing memory B cells in both CD5+ and CD5− populations was noted in children with JIA (p<0.001), whereas the number of RAG1-expressing B cells was slightly increased. The coordinate expression of both the RAG genes was a rare event, similar in the CD5+ populations (1% in controls, 2% in children with JIA), but different among the CD5− compartments (5% v 0%; p<0.01).
Conclusion: These results argue for a reduced coordinate RAG expression in the peripheral CD5− memory B cells of patients with o-JIA. Thus, it was hypothesised that impaired receptor revision contributes to autoimmune pathogenesis in JIA.
- AID, activation-induced cytidine deaminase
- ANA, antinuclear antibodies
- dUTP, deoxyuridine triphosphate
- o-JIA, oligoarticular juvenile idiopathic arthritis
- PCR, polymerase chain reaction
- RAG, recombination-activating gene
- SLE, systemic lupus erythematosus
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Published Online First 27 February 2006
Competing interests: None.