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Ann Rheum Dis 2005;64:616-619 doi:10.1136/ard.2004.027698
  • Extended report

Susceptibility to ankylosing spondylitis: no evidence for the involvement of transforming growth factor β1 (TGFB1) gene polymorphisms

  1. M van der Paardt1,
  2. J B A Crusius2,
  3. M A García-González2,
  4. B A C Dijkmans4,
  5. A S Peña3,
  6. I E van der Horst-Bruinsma4
  1. 1The Jan van Breemen Institute, VU University Medical Centre, Amsterdam, Netherlands
  2. 2Laboratory of Immunogenetics, VU University Medical Centre
  3. 3Department of Gastroenterology, VU University Medical Centre
  4. 4Department of Rheumatology, VU University Medical Centre
  1. Correspondence to:
    Dr I E van der Horst-Bruinsma
    VU University Medical Centre, Department of Rheumatology, Room 4A-42, PO Box 7057, 1007 MB Amsterdam, Netherlands; secr.reumatologievumc.nl
  • Accepted 22 August 2004

Abstract

Background: Genetic factors are thought to be crucial in the pathogenesis of ankylosing spondylitis. Transforming growth factor β1 (TGFβ1) is a multifunctional cytokine that plays a key role in inflammation. Two functional single nucleotide polymorphisms (SNPs) in the TGFB1 gene have been described: TGFB1 T869C and TGFB1 G915C.

Objective: To determine whether these SNPs contribute to ankylosing spondylitis susceptibility or its disease characteristics.

Methods: Genomic DNA was isolated from the peripheral blood of 134 patients with ankylosing spondylitis and 194 healthy blood donors. All subjects were unrelated and of white Dutch ethnicity. The diagnosis of ankylosing spondylitis was made according to the modified New York criteria. The TGFB1 T869C and TGFB1 G915C SNPs were genotyped by a polymerase chain reaction–single strand conformation polymorphism haplotyping method.

Results: No significant differences were found between patients and controls in genotype, allele, and haplotype frequencies or in the carrier rate of the rare alleles of the TGFB1 T869C and TGFB1 G915C SNPs.

Conclusions:TGFB1 T869C and TGFB1 G915C SNPs are not major factors in the susceptibility to ankylosing spondylitis or its disease characteristics.

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