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Heat shock protein 70 gene polymorphisms in Mexican patients with spondyloarthropathies
  1. G Vargas-Alarcón1,
  2. J D Londoño2,
  3. G Hernández-Pacheco1,
  4. R Gamboa1,
  5. E Castillo1,
  6. C Pacheco-Tena3,
  7. M H Cardiel2,
  8. J Granados2,
  9. R Burgos-Vargas3
  1. 1Cellular Biology Section, Department of Physiology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico DF Mexico
  2. 2Department of Immunology and Rheumatology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico DF, Mexico
  3. 3Department of Rheumatology, Hospital General de Mexico, Mexico DF, Mexico
  1. Correspondence to:
    Dr G Vargas-Alarcón, Cellular Biology Section, Department of Physiology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano No 1, Tlalpan 14080, Mexico DF, Mexico;
    gvargas63{at}yahoo.com

Abstract

Objective: To investigate the role of HSP70 genes as contributors to genetic susceptibility of the spondyloarthropathies (SpA) in the Mexican population.

Methods: The study included 150 patients with SpA (undifferentiated spondyloarthropathy (uSpA) 68, ankylosing spondylitis (AS) 60, and reactive arthritis 22) and 158 healthy controls. HSP70-1, HSP70-2 and HSP70-hom genotypes were analysed by the polymerase chain reaction-restriction fragment length polymorphism technique. Statistical methods included the Mantel-Haenzel, χ2, Fisher's exact test, and Woolf's method for odds ratio (OR).

Results: HSP70-2 B/B genotype frequency was increased in the whole group of patients with SpA (pC<0.05, OR=4.3), as well as in the different clinical subgroups (pC<0.05, OR=4.2 for AS; pC<0.05, OR=4.4 for uSpA; and pC<0.05, OR=4.1 for ReA). This frequency remained significantly increased when the patients with B27 negative SpA were analysed. On the other hand, HSP70-hom locus analysis showed significantly increased frequency of A allele in the whole group of SpA (pC<0.05, OR=3.4), as well as in the groups with AS (pC<0.05, OR=5.6) and with uSpA (pC<0.05, OR=3.1), when compared with healthy controls. In this case, also, the genotype A/A was increased in the whole group of SpA (pC<0.05, OR=4.5), as well as in patients with AS (pC<0.05, OR=6.4) and with uSpA (pC<0.05, OR=3.7). When the patients with B27 negative SpA were analysed the frequencies of HSP70-hom A allele and A/A genotype remained significantly increased in the whole group of SpA (pC<0.05, OR=3.2 for the A allele and pC<0.05, OR=4.2 for the A/A genotype) and in the uSpA subgroup (pC<0.05, OR=3.8 for the A allele and pC<0.05, OR=4.3 for the A/A genotype).

Conclusion: In addition to the association of SpA with HLA-B27, there is a significant association of HSP70-2 and HSP70-hom alleles with SpA in Mexicans. This association seems to be independent of the susceptibility conferred by HLA-B27 in the group of patients with uSpA.

  • heat shock proteins, spondyloarthropathies
  • genetic susceptibility
  • Mexican population
  • genotype
  • AF, aetiological fraction
  • AS, ankylosing spondylitis
  • HSP, heat shock protein
  • OR, odds ratio
  • PCR, polymerase chain reaction
  • ReA, reactive arthritis
  • SpA, spondyloarthropathies
  • uSpA, undifferentiated spondyloarthropathy

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