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FRI0171 Levels of soluble vcam-1, p-selectin, and e-selectin in patients with primary antiphospholipid syndrome and systemic lupus erytematosus
  1. AA Novikov,
  2. EN Alexandrova,
  3. TM Reshetnyak
  1. Clinical Immunology, Cardiology Research Center, Moscow, Russia

Abstract

Background The antiphospholipid antibodies (aPL) are known to modify the expression of adhesion molecules on activated endothelial cells and platelets in vitro and in vivo.

Methods We studied the levels of soluble (s) P-selectin, sE-selectin, and sVCAM-1 by ELISA in sera from 19 patients (pts) (5 male, 14 female, mean age? 36.9 ± 10.1 years) with primary antiphospholipid sindrome (PAPS), 23 pts (10 male, 13 female, mean age ? 35.3 ± 11.6 years) with systemic lupus (SLE) erythematosus? related antiphospholipid sindrome (SLE-APS), 15 pts (13 male, 2 female, mean age? 29.0 ± 9.0 years) with SLE and 38 controls.

Results The sE-selectin and sVCAM-1 concentrations were significantly higher in pts with PAPS, SLE-APS and SLE compared with controls. (78.3 ± 61.2 ng/ml; 52.3 ± 14.1 ng/ml; 70.5 ± 46.9 ng/ml vs 38.0 ± 13.4 ng/ml; p = 0.0019, p = 0.0363, p = 0.0144, respectively for sE-selectin; 1003 ± 582.6 ng/ml; 1189.6 ± 691.0 ng/ml; 1227 ± 680.2 ng/ml vs 594.7 ± 63.3 ng/ml; p = 0.0001 for sVCAM-1).

The sP-selectin concentration was significantly higher in pts with SLE compared with controls (262.4 ± 158.8 ng/ml vs 120.1 ± 53.7 ng/ml; p = 0.0037). Within the group of pts with PAPS the sP-selectin concentration was higher among those with ischaemic CNS involvement. (p < 0.02).

The serum C-reactive protein level was positively correlated with the sE-selectin concentration in SLE-APS (r = 0.341, p < 0.05) and SLE (r = 0.699, p < 0.01), the sVCAM-1 concentration in PAPS (r = 0.705, p < 0.001) and SLE (r = 0.405, p < 0.05).

The serum, anti ds and ssDNA antibody level was positively correlated with the sP-selectin concentration in SLE-APS (r = 0.730, and r = 0.909, p < 0.001), and the sE-selectin concentration in SLE (r = 0.684, p < 0.001 and r = 0.616, p < 0.002).

Conclusion The sE-selectin and sVCAM-1 concentrations were significantly higher in pts with PAPS, SLE-APS and SLE compared with controls. (78.3 ± 61.2 ng/ml; 52.3 ± 14.1 ng/ml; 70.5 ± 46.9 ng/ml vs 38.0 ± 13.4 ng/ml; p = 0.0019, p = 0.0363, p = 0.0144, respectively for sE-selectin; 1003 ± 582.6 ng/ml; 1189.6 ± 691.0 ng/ml; 1227 ± 680.2 ng/ml vs 594.7 ± 63.3 ng/ml; p = 0.0001 for sVCAM-1).

The sP-selectin concentration was significantly higher in pts with SLE compared with controls (262.4 ± 158.8 ng/ml vs 120.1 ± 53.7 ng/ml; p = 0.0037). Within the group of pts with PAPS the sP-selectin concentration was higher among those with ischaemic CNS involvement (p < 0.02).

The serum C-reactive protein level was positively correlated with the sE-selectin concentration in SLE-APS (r = 0.341, p < 0.05) and SLE (r = 0.699, p < 0.01), the sVCAM-1 concentration in PAPS (r = 0.705, p < 0.001) and SLE (r = 0.405, p < 0.05).

The serum, anti ds and ssDNA antibody level was positively correlated with the sP-selectin concentration in SLE-APS (r = 0.730, and r = 0.909, p < 0.001), and the sE-selectin concentration in SLE (r = 0.684, p < 0.001 and r = 0.616, p < 0.002).

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