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FRI0089 Il10G promoter polymorphism of the interleukin 10 (il10) in spanish systemic lupus erythematosus (sle) patients
  1. S Rosado-Garcia1,
  2. MJ Citores1,
  3. T Martin-Donaire1,
  4. I Rua-Figueroa2,
  5. I Garcia-Laorden3,
  6. C Rodriguez-Lozano2,
  7. P Perez-Aciego1,
  8. C Rodriguez-Gallego3,
  9. A Durantez4
  1. 1Laboratorio, Fundacion Lair
  2. 2Reumatologia
  3. 3Inmunologia, Hospital de Gran Canaria Doctor Negrin, Las Palmas de Gran Canaria, Spain
  4. 4Medicina Interna I, Clinica Puerta de Hierro, Madrid

Abstract

Background IL10 is a pleiotrophic cytokine that inhibits the antigen specific activation of Th1 clones by reducing both the antigen presenting capacity and the production of pro-inflammatory cytokines by monocytes. It is also a potent activation and differentiation factor for B lymphocytes. SLE patients show increased IL10 serum levels associated with the pathogenesis of the disease. Peripheral blood mononuclear cells from SLE patients, and their relatives, constitutively produce high levels of the cytokine, suggesting an hereditary component in IL10 production. Anti-IL10 antibodies administration to SLE patients may be beneficial in the management of refractory cases. The IL10 gene promoter contains two dinucleotide repeats (microsatellites): IL10G and IL10R, close to transcription factor binding sites, probably playing an important role in the transcription regulation.

Objectives To evaluate the association of the IL10G microsatellite with SLE in Spanish patients.

Methods 80 unrelated SLE patients from Canary Islands (Spain) fulfilling at least four of the ACR criteria and 79 sex-matched healthy donors were included. A segment of the IL10 gene containing the microsatellite was amplified by PCR and the resulting products were electrophoresed in denaturing polyacrilamide gels. Chi-square test was used for statistical analysis.

Results No particular IL10G allele was found to be significantly associated with SLE. Neverthaless, we found an elevated risk of serositis in carriers of the allele 19 (p = 0.007), a significant association of the 19/23 genotype with seizures (p = 0.018), and a reduced risk of renal disorder (p = 0.047) and thrombocytopenia (p = 0.049) in carriers of the allele 23.

Conclusion Although IL10G alleles seem not to influence susceptibility to SLE in Spaniards, some of them may be related to the presence of several clinical manifestations of the disease.

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