Objectives To investigate the effect of interleukin-17 (IL-17) on production of matrix metalloproteinase-3 (MMP-3) in rheumatoid arthritis synoviocytes.
Methods Fibroblast-like synovial cell (FLS) were prepared from the synovial tissue of rheumatoid arthritis patients and cultured in the presence of various concentration of IL-17. MMP-3 levels were determined in the culture supernatants by sandwitch ELISA according to culturing periods.
Results Stimulation of FLS by IL-17 with 5, 10, 20, 40 ng/ml concentrations increased the production of MMP-3 by 2.7, 2.7, 3, 3.4 fold after 24 h and 8.7, 8.7, 8.1, 10.2 fold after 48 h over the constitutive levels of unstimulated FLS.
Conclusion IL-17, a T cell-derived proinflammatory cytokine produced by rheumatoid synovium, may be involved in the cartilage degradation in rheumatoid arthritis by enhancing the production of MMP-3 from rheumatoid arthritis synoviocytes.
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