Objectives Purpose of research was to study presence and role of definition of antibodies to 5’nucleotidase (anti-5NT) in rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), systemic sclerosis (SS), ankylosing spondylarthritis (AS) and reactive arthritis (ReA) patients.
Methods There were 100 RA, 30 SLE, 20 SS, 30 AS and 20 ReA patients and 30 healthy donors (HD) under our observation. Anti-5NT were determined in serum of a blood with ELISA-test. We used 5’nucleotidase (AN 220.127.116.11) from Crotalus adamenteus venom («Sigma») as antigen.
Results We revealed, that the anti-5NT level in HD was equal 0,048 ± 0,004, in RA – 0,153 ± 0,012, in SLE – 0,101 ± 0,016, in SS – 0,117 ± 0,017, in AS 0,118 ± 0,015, in ReA – 0,117 ± 0,010 (in all cases p < 0.05). We revealed difference between anti-5NT level in RA and SLE (p < 0,05), that can be used in differential diagnostics of these diseases. Was noted increasing of anti-5NT level in RA with muscles injury (without myositis – 0,150 ± 0,013, with myositis – 0,190 ± 0,002) and kidney manifestations (without nephropathy – 0,149 ± 0,013, with nephropathy – 0,205 ± 0,013). Taking into account that kidney injury in RA associates with perinuclear antineutrophil cytoplasmic antibody, we assume presence of cross reactions between them and anti-5NT.
Conclusion Thus, we revealed the new group of autoantibodies. The determination of them can improve diagnostics and differential diagnostics and better understanding of rheumatic diseases pathogenesis.
Mustila A, Korpela M, Mustonen J, et al. Perinuclear antineutrophil cytoplasmic antibody in rheumatoid arthritis: a marker of severe disease with associated nephropathy. Arthritis Rheum. 1997;40(4):710–17
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