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AB0160 The influence of different kinds of theraphy on oxidative-antioxidative balance in children with juvenile idiopathic arthritis/jia/
  1. J Postêpski,
  2. V Opoka-Winiarska,
  3. E Tuszkiewicz-Misztal,
  4. M Wawrzyszuk
  1. Department of Paediatrics, Pulmonary Diseases and Rheumatology, Medical University, Lublin, Poland

Abstract

Background The aim of this study was to investigate the influence of different kinds of theraphy on oxidative-antioxidative balance in children with rheumatoid idiopatic arthritis.

Objectives In our study the intensity of peroxide lipid oxidation was calculated by the contents of malonic dialdehyde (MDA) in plasma. At the same time antioxidant activity was evaluated. We measured the content of vitamin E (Vit. E) in plasma, the total antioxidant status (TAS), as well as the activity of glutathion peroxidase (GPX) and superoxide dismutase (SOD).

In our study we examined 39 children with JIA aged 4–18 years and 27 healthy subjects aged 4–16 years.

8 patients were treated with glicocorticosteroids administered 1–2 mg/kg/24 h in relation to the prednisone standard. 8 children received Methtrexatein the dose of 10–15 mg/m2/week. 9 children were treated with Sulphasalazine in the dose of 20–40 mg/kg/24 h. 14 children were treated with non-steroid anti-inflammatory agents (naproxen 10 mg/kg/24 h). The examination was performed 4–6 weeks after the beginning of the treatment. The results obtained after the treatment were compared with those obtained before the treatment and those in the control group.

Methods We used colorymetric and fluorometric methods.

Results The level of MDA before the treatment, i.e. 2,32 ± 0,86 nM/ml, was significantly higher than that in the control group, i.e. 1,32 ± 0,38 nM/ml (p < 0,05). After the treatment with Mtx, Sulphasalazine and non-steroid anti-inflammatory agents, the values of MDA were still significantly higher than those in the control group and equalled, respectively, 2,32 ± 0,71 nM/ml, 2,14 ± 0,1 nM/ml, and 2,14 ± 0,1 nM/ml (p < 0,05). After the treatment with glicocorticosteroids, the values of MDA approximated to those in the control group and averaged 1,72 ± 0,17 nM/ml (p < 0,05). Before the treatment, the activity of GPX was significantly lower than those in the control group (4,75 ± 2,59 U/ml vs 7,06 ± 1,99U/ml (p < 0,05)). The values of the other parameters did not appear to depend on the treatment.

Conclusion 1. Our results confirmed the existence of oxidative stress in children with juvenile idiophatic arthritis. 2. The strongest inhibitory effect on peroxide lipid oxidation is exerted by glicocorticosteroids, which conforms to their strongest anti-inflammatory activity. No significant influence of non-steroid anti-inflammatory agents on peroxide lipid oxidation was observed, despite their generally-acknowledged, weak antioxidative activity. No influence of Mtx and Sulphasalazine on peroxide lipid oxidation was observed in the period of examination.

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