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SAT0047 Purine metabolism and antioxidant system in osteoarthritis
  1. AB Zborovsky,
  2. MY Stazharov,
  3. VF Martemyanov,
  4. SA Bedina,
  5. EE Mozgovaya,
  6. TP Chernykh
  1. Laboratory for Clinical Biochemistry, Research Institute for Clinical and Experimental Rheumatology, Volgograd, Russia

Abstract

Background Till now there are few of information on a Purine metabolism (PM) and Antioxidant system (AS) condition of blood of osteoarthritis (OA) patients.

Objectives To study the enzymatic profile of PM and AS in blood of OA patients.

Methods The activities of Xanthine Oxidase (XO), Xanthine dehydrogenase (XDG), 5’-Nucleotidase (5’-NT), Adenosindesa-minase (ADA), Guanindesaminase (Guanase), Superoxid-dismutase (SOD), Glutathion reductase (GP), the contents of Uric acid (UA) were measured with Spectrophotometer. XDG, ADA, SOD isoenzymes were examined with method of electrophoresis in agarose gel and obtained enzymograms were evaluated at Densitometry.

Results 44 patients with OA and 30 healthy controls (HC) were under observation. The mean age of patients – 53 ± 2.6 years. The mean duration of disease – 10.2 ± 2.7 years. We revealed the increase of XO, ADA, Guanasa, SOD, GP, GR activity, XDG-2, SOD-1 isoensymes, contents of UA in OA patients with synovitis in comparison with HC. The increase of XDG, SOD, GP activity, SOD-1 isoensymes were observed in OA patients without synovitis in comparison with HC and the decrease of UA, XDG-2 isoenzymes, also the increase of XDG, GP activity were revealed in comparison with OA patients with synovitis.

Conclusion PM and AS are essentially changed in OA. These changes depended on the clinical form of OA. The enzyme indices can be used for early activity of disease diagnostic and differentiation of OA clinical form.

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