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SAT0001 Role of determination of succinate dehydrogenase, myeloperoxidase, na ± k ± atph-ase, 5?-nucleotidase in cells of peripheral blood of ankylosing spondylarthritis patients
  1. AB Zborovsky,
  2. BV Zavodovsky,
  3. TA Pankratova,
  4. AV Rvachev,
  5. OV Bykova,
  6. TV Serdukova
  1. Laboratory for Arthritis and Arthrosis, Research Institute for Clinical and Experimental Rheumatology, Volgograd, Russia

Abstract

Objectives To assess the value of determination Myeloperoxidase (MP), Na ± K ± AOPh-ase (ATPh-ase), 5’-Nucleotidase (5?-NT), Succinate dehydrogenase (SDG) in cells of peripheral blood of ankylosing spondylarthritis (AS) patients.

Methods We observed 30 AS patients and 30 healthy donors (HD) as control group. The activities of enzymes were determined by hystochemical methods.

Results We observed increasing of ATPh-ase in neutrophils and lymphocytes, MP in monocytes, 5?-NT in lymphocytes, monocytes and neutrophils, decreasing of MP in neutrophils (p < 0,05). We observed the greatest increasing of ATP-ase in neutrophils in 1st degree of AS activity, 5?NT in all cells and MP in monocytes – in fast progressing AS. Level of SDG in lymphocytes in AS did not differ from HD. There were correlation between level MP in neutrophils and expressiveness of an anaemia (r = -0,41, p = 0,029). Reliable dynamics of enzymes activities were noted during the course of treatment. We revealed normalisation of MP in monocytes and neutrophils when the patients received indomethacin. There were decreasing of 5?NT level at the treatment by NSAIDs and steroid hormones. There was correlation of clinical improvement with activity of 5?NT in blood cells.

Conclusion The determination of MP, ATPh-ase, 5?-NT, but not SDG activities in blood cells can be used for improvement of quality of AS diagnostics and for control of treatment quality.

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