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FRI0216 Antibodies to recombinant 60-kd ro, 52-kd ro and 48-kd la proteins in primary sjÖgren’s syndrome. usefulness of combination of analytical methods for detecting anti-ro and anti la antibodies
  1. S Aguilera1,
  2. D Castillo2,
  3. J González3
  1. 1Rheumatology
  2. 2Inmunology
  3. 3Department of Cellular and Molecular Biology, University of Chile, Santiago, Chile

Abstract

Objectives To determine the reactivity and specificity of sera from primary Sjögren’s syndrome (SS) patients to recombinant 60-Kd Ro, 52-Kd Ro and 48-Kd La proteins, and to correlate the results with clinical and laboratory findings.

Methods Sera from 46 patients with primary SS diagnosed according to modified 1997 European criteria, were classified in 2 groups based on the presence of anti- Ro and anti-La detected by commercial enzyme linked immunobsorbent assay (ELISA). Group A (n = 32) had anti-Ro positive sera, and group B (n = 14) had anti-Ro and anti-La negative sera, but positive antinuclear antibodies and/or rheumatoid factors. Autoantibody reactivity and specificity to recombinant 60-Kd Ro, 52-Kd Ro and 48-Kd La were analysed by ELISA and Western blot.

Results Forty-two out of 46 primary SS sera (91.30%), had reactivity to either natural or recombinant antigens or both. Anti-60-Kd Ro was found in 27 (58.69%) of all sera, of these, 22 (68.75%) sera were of group A, and 5 sera (35.71%) of group B. Anti-52-Kd Ro was highly prevalent since 38 out of 46 sera (62.60%) were positive. Among group A, 31 out of 32 (96.87%) sera were positive for 52-Kd Ro, and 7 out of 14 (50%) in group B. Anti-recombinant 48-Kd La was also highly prevalent, 38 (82.60%) of all sera were positive. In group A, 31 (96.87%) of sera were positive, while 5 (35.71%) of sera of group B, were positive. Ten (71.42%) of 14 patients of group B had antibodies for at least 1 of 3 recombinant antigens: 52-Kd Ro, 60-Kd Ro or 48-Kd La. Comparison of ELISA and Western blot produced a good intermethod agreement coefficient when anti-recombinant antibodies from sera in group A were tested: 60-Kd Ro (k = 0.563) and 52-Kd Ro (k = 0.652). Both groups were similar regarding of baseline characteristics, except that patients in group A who were younger at the time of diagnosis than those of group B (mean 39.9 v/s 63.7 years), and hipergammaglobulinemia was more frequent in group A than B. Extraglandular manifestations were similar in both groups.

Conclusion The detection of antibodies to recombinant 60-Kd Ro, 52-Kd Ro and 48-Kd La proteins, might be a new research tool as well as an aid for the diagnosis of SS, mainly in cases who are seronegative for anti-Ro or La by conventional techniques.

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