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Bacterial PCR in the diagnosis of joint infection
  1. J Jalavaa,
  2. M Skurnikb,
  3. A Toivanenc,
  4. P Toivanena,
  5. E Eerolaa
  1. aDepartment of Medical Microbiology, University of Turku, bDepartment of Medical Biochemistry, University of Turku, cDepartment of Medicine, University of Turku
  1. Dr J Jalava, National Public Health Institute, Turku, Kiinamyllynkatu 13, FIN-20520, Turku, Finlandjari.jalava{at}utu.fi

Abstract

OBJECTIVES To evaluate the value of broad range bacterial PCR in the diagnosis of joint infection and to find out if there are bacteria causing arthritis which are not cultivable by the present methods.

METHODS Polymerase chain reaction (PCR) with broad range bacterial primers and DNA sequencing (bacterial PCR) was used to analyse 154 synovial fluid (SF) samples from patients with different arthritic diseases.

RESULTS Bacterial DNA was detected in 18 SF samples, including samples from six patients with culture proven purulent arthritis, and from three patients with possible purulent arthritis. Three samples from patients with culture confirmed purulent arthritis remained negative in bacterial PCR.

CONCLUSIONS The results indicate that in the usual diagnostic laboratory setting bacterial PCR does not offer any obvious advantage over bacterial culture in the microbiological diagnosis of joint infection.

  • polymerase chain reaction
  • joint infection

https://doi.org/10.1136/ard.60.3.287

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