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Ann Rheum Dis 1999;58:96-102 doi:10.1136/ard.58.2.96
  • Extended reports

A quantitative method for detecting deposits of amyloid A protein in aspirated fat tissue of patients with arthritis

  1. Bouke P C Hazenberg,
  2. Pieter C Limburg,
  3. Johan Bijzet,
  4. Martin H van Rijswijk
  1. Division of Rheumatology, Department of Medicine, University Hospital, Groningen, the Netherlands
  1. Dr B PC Hazenberg, Department of Rheumatology, University Hospital, PO Box 30.001, 9700 RB Groningen, the Netherlands.
  • Accepted 23 October 1998

Abstract

OBJECTIVE To describe a new, quantitative, and reproducible method for detecting deposits of amyloid A protein in aspirated fat tissue and to compare it with smears stained with Congo red.

METHODS After extraction of at least 30 mg of abdominal fat tissue in guanidine, the amyloid A protein concentration was measured by a monoclonal antibody-based sandwich ELISA.

RESULTS The concentrations in 24 patients with arthritis and AA amyloidosis (median 236, range 1.1–8530 ng/mg tissue) were higher (p<0.001) than in non-arthritic controls, uncomplicated rheumatoid arthritis, and other types of systemic amyloidosis (median 1.1, range 1.1–11.6 ng/mg tissue). Patients with extensive deposits, according to Congo red staining, had higher concentrations than patients with minute deposits.

CONCLUSION This is a new, quantitative, and reproducible method for detecting deposits of amyloid A protein in aspirated fat tissue of patients with arthritis, even when minute deposits are present as detected in smears stained with Congo red.

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