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Ann Rheum Dis 1998;57:487-494 doi:10.1136/ard.57.8.487
  • Extended reports

Involvement of simultaneous multiple transcription factor expression, including cAMP responsive element binding protein and OCT-1, for synovial cell outgrowth in patients with rheumatoid arthritis

  1. Sueshige Wakisakaa,
  2. Noboru Suzukia,
  3. Mitsuhiro Takenoa,
  4. Yuko Takebaa,
  5. Hiroko Nagafuchia,
  6. Nobuhiko Saitob,
  7. Hideo Hashimotoc,
  8. Tetsuya Tomitac,
  9. Takahiro Ochic,
  10. Tsuyoshi Sakanea
  1. aDepartments of Immunology and Medicine, band Department of Internal Medicine, cSt Marianna University School of Medicine, Kawasaki, Kanagawa, Japan Department of Orthopaedic Surgery, Osaka University School of Medicine, Osaka, Japan
  1. Dr T Sakane, Departments of Immunology and Medicine, St Marianna University School of Medicine, 2–16–1, Sugao, Miyamae-ku, Kawasaki, Kanagawa 216, Japan.
  • Accepted 21 May 1998

Abstract

OBJECTIVE To elucidate possible roles of several transcription factors in the pathogenesis of rheumatoid arthritis (RA), the transcription factor expression in RA synovial tissue and their contribution to RA synovial cell functions were studied.

METHODS Single cell suspension of dissociated synovial tissue was cultured to induce in vitro tissue outgrowth of RA synovial cells. Transcription factors were immunohistochemically identified in RA synovial tissue obtained by joint surgery and in the in vitro tissue outgrowth, and confirmed by western blotting and gel shift assays.

RESULTS Immunohistochemical examination of RA synovial tissue revealed simultaneous expression of various transcription factors (NF-κB, c-Jun (a component of AP-1), cAMP responsive element binding protein (CREB), and OCT-1). The same set of transcription factors was expressed in the in vitro tissue outgrowth of RA patients. The early passage RA synovial cells were treated with interleukin 1β (IL1β) and confirmed translocation of transcription factors into the nucleus by western blotting, and their DNA binding activity by gel shift assays.

CONCLUSION This study emphasises the importance of the simultaneous expression of several transcription factors for the hyperactivity of RA synovial cells that leads to tissue outgrowth.

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