The LMP2 polymorphism is associated with susceptibility to acute anterior uveitis in HLA-B27 positive juvenile and adult Mexican subjects with ankylosing spondylitis
- Walter P Maksymowycha,
- Gian S Jhangrib,
- Clara Gorodezkyd,
- Maria Luonga,
- Cindy Wonga,
- Rubén Burgos-Vargasc,
- Monica Morenotd,
- José Sanchez-Coronae,
- César Ramos-Remusf,
- Anthony S Russella
- aDepartments of Medicine , band Public Health Sciences , cUniversity of Alberta, Canada Rheumatology Unit, Hospital General de Mexico, Mexico City, dDepartment of Immunogenetics, INDRE, SSA, Mexico City , eCentro de Investigacion Biomedica, IMSS, Guadalajara , fDepartment of Rheumatology, Hospital de Especialidades CMNO, IMSS, Guadalajara, Mexico
- Dr W P Maksymowych, 562 Heritage Medical Research Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2S2.
- Accepted 6 June 1997
Abstract
INTRODUCTION An association between polymorphism of the HLA linked LMP2 locus and the development of acute anterior uveitis (AAU) has previously been described in B27 positive white subjects with ankylosing spondylitis (AS). This study evaluated LMP2 alleles in two HLA-B27 positive Mexican populations of patients with spondyloarthropathy known to have a different clinical spectrum of disease from white people.
PATIENTS AND METHODS The study populations consisted of 90 AS patients from Guadalajara with predominantly adult onset disease and 80 AS patients from Mexico City with predominantly juvenile onset disease. LMP2-CfoI amplified fragment length polymorphisms were determined after polymerase chain reaction amplification and digestion with CfoI restriction enzyme.
RESULTS There was an increased LMP2A allelic frequency in patients who had had AAU in both Guadalajara (31.8%) and Mexico City (33.3%) when compared with non-AAU patients (15.2% and 17.7% of Guadalajara and Mexico City populations, respectively). The odds ratio relating LMP2A allelic frequency and AAU for the combined population, stratified by age at onset of disease, was 2.51 (p=0.01). LMP2 alleles did not influence the age at onset of disease or the development of peripheral arthritis.
CONCLUSIONS These data support the view that polymorphism at the LMP2 locus is associated with the development of AAU in B27 positive subjects with AS. The requirement for both the less common LMP2 allele and HLA-B27 is consistent with the low prevalence of AAU in Mexican patients with spondyloarthritis.
