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Synovial fluid hydroxyapatite crystals: detection thresholds of two methods.
  1. T Cunningham,
  2. D Uebelhart,
  3. J M Very,
  4. G H Fallet,
  5. T L Vischer
  1. Division of Rheumatology, Hôpital Cantonal Universitaire, Geneva, Switzerland.


    A method of synovial fluid preparation giving optimal hydroxyapatite detection as well as definitions of the threshold masses of hydroxyapatite in viscous synovial fluid detectable by x ray diffraction and scanning electron microscopy with energy dispersive analysis is reported. Use of an equal volume of 100% hydrazine with the synovial fluid optimised detection of hydroxyapatite. By x ray diffraction the threshold mass of hydroxyapatite was 500 micrograms and by scanning electron microscopy with associated energy dispersive analysis 5 micrograms.

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