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Research Article
Use of cultured epithelial cells, including keratinocytes, for the detection of antinuclear antibodies.
  1. I Leigh,
  2. F Wojnarowska,
  3. S Burge,
  4. B Bhogal,
  5. T Zhu
  1. Department of Dermatology, London Hospital.

    Abstract

    The use of cultured human epithelial cells as a substrate for the detection of antinuclear antibodies (ANAs) improves the sensitivity of detection above the standard substrates used. Standard antisera have been screened against a series of epithelial cell lines, including keratinocytes. Selected cells have then been used to detect ANAs in 78 patients with discoid lupus erythematosus.

    https://doi.org/10.1136/ard.46.12.929

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