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Synthesis of arachidonate cyclo-oxygenase products by rheumatoid and nonrheumatoid synovial lining in nonproliferative organ culture
  1. J. A. Salmon1,
  2. G. A. Higgs1,
  3. J. R. Vane1,
  4. Lucille Bitensky2,
  5. J. Chayen2,
  6. B. Henderson2,
  7. B. Cashman3
  1. 1Department of Prostaglandin Research, Wellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3 BS
  2. 2Division of Cellular Biology, Kennedy Institute of Rheumatology, Bute Gardens, London W6 7DW
  3. 3Orthopaedics Department, Bedford General Hospital, Bedford


    Specimens of human rheumatoid and nonrheumatoid synovial lining were maintained in nonproliferative organ culture for 20 hours. The culture fluids were then assayed for prostaglandin E2 (PGE2), thromboxane B2 (TXB2), and 6-keto-prostaglandin F (6-keto-PGF) by specific radioimmunoassay. The presence of each of these substances was confirmed by gas chromatography and mass spectrometry. Rheumatoid tissue produced significantly more of each cyclo-oxygenase product than nonrheumatoid tissue.

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