Article Text

PDF

Type C oncornavirus isolation studies in systemic lupus erythematosus
  1. Paul E. Phillips,
  2. Rita Hargrave,
  3. Elaine Stewart,
  4. Nurul H. Sarkar
  1. Departments of Medicine, Hospital for Special Surgery, New York
  2. Memorial-Sloan-Kettering Cancer Center, and Cornell University Medical College, New York

    Abstract

    Phillips, P. E., Hargrave, R., Stewart, E., and Sarkar, N. H. (1976).Annals of the Rheumatic Diseases, 35, 422-428. Type C oncornavirus isolation studies in systemic lupus erythematosus. I. Attempted detection by isopycnic sedimentation of 3H-uridine-labelled virions. Type C oncornavirus isolation was attempted from cell cultures of tissues from 7 patients with systemic lupus erythematosus. Detection was based on the characteristic sedimentation of 3H-uridine-labelled virions at a density of 1·16 g/ml. Cultures positive by this method were negative by two other criteria for type C viruses: characteristic virions by electron microscopy and the viral enzyme RNA-directed DNA polymerase. The positive results were probably due to cellular damage by prolonged radiolabelling, with release of organelles containing labelled RNA sedimenting at the same density as type C viruses.

    Statistics from Altmetric.com

    I. Attempted detection by isopycnic sedimentation of 3H-uridine-labelled virions

    Request permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.