Web-only Figures

Three web-only figures accompany this article. They are provided below.

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• [View Figure] - Figure W1 Progression of articular cartilage degeneration in knee joints of transgenic Del1 mice and nontransgenic controls. Grading of osteoarthritic changes was performed as described earlier. 5 33 34 Closed symbols denote Del1 samples and open circles their control litter mates. (Mann-Whitney U test; n = 5–30; *** p<0.001)
• [View Figure] - Figure W2 Temporal expression of cysteine cathepsins and cystatin C mRNAs in developing, growing and aging mouse knee joints. Total RNAs were extracted from entire knee joints of Del1 and control mice at birth and at the age of 4 and 9 months (marked above lines; nb, newborn; mo, month; , control; +, Del1). The hybridisations were performed with cDNA probes detecting mRNAs for cathepsin B (Ctsb), cathepsin H (Ctsh), cathepsin K (Ctsk), cathepsin L (Ctsl) and cathepsin S (Ctss), as well as for cystatin C (Cst3) (marked on the left, respectively). The lowest panel 28S demonstrates the hybridisation of the filter with a probe for 28S rRNA. Hybridisation intensity was analysed using a phosphoimager. Transcript sizes are shown on the right.
• [View Figure] - Figure W3 Immunohistochemical detection of cathepsin K and cystatin C in the mouse synovial tissue at the age of 4 months. Tissue distribution is shown in sagittal sections of knee joints obtained from control (A, D, F) and Del1 mice (B, C, E). Immunostaining for cathepsin K (A, B) and cystatin C (D, E) is identified as a brown precipitate. There was no staining for cathepsin K (C) or cystatin C (F) when primary antibodies were omitted or replaced by normal mouse serum. The bar in panel A corresponds to 15 m for panels A-F. Paraffin sections; biotin-streptavidin complex with horseradish peroxidase label and diaminobenzidine detection, counterstaining with hematoxylin.