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• [View PDF] - Figure W1. Additional serial sections of normal and OA cartilage are demonstrated. A Healthy articular cartilage with a smooth surface and a high content of proteoglycans (safranin-o staining). B Tenascin immunostaining reveals tenascin deposition at the surface lining, in the superficial zone and middle zone in a territorial and interterritorial staining pattern (normal cartilage with the strongest staining found in our study, same specimen shown in A). C Mild OA lesion with pannus-like tissue on the surface (safranin-o staining). D Immunohistochemical analysis of tenascin in the same section shown in C (note the strong immunostaining of pannus-like tissue indicated by arrows). E Moderate OA cartilage lesion with a reduction of proteoglycans, loss of the superficialzone, fissuring of the surface and chondrocyte clusters next to the surface (safranin-o staining). F Immunohistochemical detection of tenascin in the same section shown in E (note the strong tenascin staining in cartilage areas with a strong reduction of safranin-o staining indicated by arrowheads). G Proteoglycan staining of severely affected cartilage with great reduction of cartilage substance and small clusters next to the joint space. H Immunostaining for tenascin revealed a strong tenascin deposition in the upper OA cartilage (note the strong tenascin staining in cartilage areas with a total loss of proteoglycans indicated by arrows). Bar in A represents 100ƒÝm in A-F and 200ƒÝm in G-H.