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Transdifferentiation of polymorphonuclear neutrophils to dendritic-like cells at the site of inflammation in rheumatoid arthritis: evidence for activation by T cells

C Iking-Konert¹, B Ostendorf¹, O Sander¹, M Jost², C Wagner³, L Joosten⁴, M Schneider¹ and G M Hänsch⁵

¹ Centre for Rheumatology, Department of Endocrinology, Diabetology and Rheumatology, Heinrich-Heine-University Duesseldorf, Germany
² Department of Rheumatology, Evangelisches Fachkrankenhaus Ratingen, Germany
³ Klinik für Unfall- und Wiederherstellungschirurgie, Berufsgenossenschaftliche Unfallklinik Ludwigshafen, Germany
⁴ Rheumatology Research Laboratory and Advanced Therapeutics, Department of Rheumatology, University Medical Centre Nijmegen, The Netherlands
⁵ Professor for Immunology, Institute for Immunology, Ruprecht-Karls University of Heidelberg, Germany

Correspondence to:
Correspondence to:
Dr C Iking-Konert
Centre for Rheumatology Duesseldorf, Heinrich-Heine-University Duesseldorf, Moorenstr 5, 40225 Düsseldorf, Germany; Iking-Konert{at}med.uni-duesseldorf.de

Objectives: To investigate infiltrated cells in the synovial fluid (SF) of inflamed joints of patients with rheumatoid arthritis (RA), with special reference to polymorphonuclear neutrophils (PMN) and their interaction with T cells.

Methods: Expression on PMN of activation associated receptors CD14, CD64, CD83, and major histocompatibility complex (MHC) class II was examined in the SF of 15 patients with RA, as were the infiltrated T cells. SF cytokines were determined by enzyme linked immunosorbent assay (ELISA). To mimic the in vivo situation, co-culture experiments were carried out using PMN and T cells of healthy donors.

Results: The SF contained activated T lymphocytes and abundant PMN. SF PMN expression of CD14 and CD64 was enhanced compared with peripheral blood. Of special interest was the observation that only the SF PMN expressed MHC class II antigens and CD83. Exposure to SF, which contained considerable amounts of cytokines (for example, interferon (IFN), tumour necrosis factor , and interleukin 2), induced a similar receptor pattern on blood derived PMN of healthy donors. Furthermore, PMN acquired MHC class II and CD83 within 24 to 48 hours, when co-cultured with autologous T cells or T cell lines. This effect was also achieved by T cell supernatants, was dependent on protein synthesis, and could be inhibited by antibodies against IFN.

Conclusions: SF PMN from patients with RA undergo major alterations, including transdifferentiation to cells with dendritic-like characteristics, probably induced by T cell derived cytokines. Because MHC class II positive PMN are known to activate T cells, the mutual activation of PMN and T cells might contribute to the perpetuation of the local inflammatory process, and eventually to the destructive process in RA.

Abbreviations: ELISA, enzyme linked immunosorbent assay; FITC, fluorescein isothiocyanate; GM-CSF, granulocyte monocyte-colony stimulating factor; IFN, interferon ; MHC, major histocompatibility complex; NHD, normal healthy donors; PBS, phosphate buffered saline; PE, phycoerythrin; PMN, polymorphonuclear neutrophils; RA, rheumatoid arthritis; SF, synovial fluid; SN, supernatant(s); TNF, tumour necrosis factor

Keywords: polymorphonuclear neutrophils; CD83; MHC class II; rheumatoid arthritis; inflammation

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