CONCISE REPORT

Transforming growth factor ß1 and insulin-like growth factor 1 block collagen degradation induced by oncostatin M in combination with tumour necrosis factor from bovine cartilage

W Hui, T Cawston, A D Rowan

Department of Rheumatology, Medical School, University of Newcastle, Newcastle Upon Tyne, NE2 4HH, UK

Correspondence to:
Correspondence to:
Dr A D Rowan;
a.d.rowan{at}ncl.ac.uk

Oncostatin M (OSM) in combination with tumour necrosis factor (TNF) promotes marked collagen breakdown from bovine cartilage in explant culture. This release was dependent upon matrix metalloproteinases and could be prevented by transforming growth factor ß1 (TGFß1) or insulin-like growth factor 1. Both growth factors reduced the expression and secretion of collagenase enzymes, and TGFß1 induced tissue inhibitor of metalloproteinase production. This study shows for the first time that these anabolic growth factors can protect cartilage against OSM+TNF induced destruction.

Keywords: growth factors; cytokines; collagen; metalloproteinases

Abbreviations: DMEM, Dulbecco’s modification of Eagle’s medium; IGF, insulin-like growth factor; IL, interleukin; MMPs, matrix metalloproteinases; OSM, oncostatin M; RA, rheumatoid arthritis; TGF, transforming growth factor; TIMPs, tissue inhibitors of metalloproteinases; TNF, tumour necrosis factor

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