Concise report
Clonal analysis of B cells in the osteoarthritis synovium
S Shiokawaa, N Matsumotob, J Nishimuraa
a Department
of Clinical Immunology, Medical Institute of Bioregulation, Kyushu
University, Tsurumihara 4546, Beppu, Oita 874-0838, Japan, b Department of
Orthopaedic Surgery, National Beppu Hospital, Uchikamado 1473, Beppu,
Oita 874-0011, Japan
Correspondence to: Dr Shiokawa sahasu{at}tsurumi.beppu.kyushu-u.ac.jp
Accepted for publication 17 November
2000
OBJECTIVES
Cellular
and humoral immunity to collagen and cartilage proteoglycan were shown
in patients with osteoarthritis (OA). Inflammatory infiltration
containing T and B lymphocytes and macrophages, which are HLA-DR
positive, is often seen in the synovial membrane of patients with OA.
An analysis of the DNA restriction enzyme patterns of T lymphocytes
from the OA synovium showed an oligoclonal pattern of T cell receptor
chain gene rearrangements. No similar studies of B cell clonality
have previously been performed. This study aimed at determining the
clonal characteristics of the B cells in the OA synovium.
METHODSA reverse
transcriptase-polymerase chain reaction of the immunoglobulin
transcripts of B cells in the synovial membranes from six patients with
OA was performed and the products were analysed by a single strand
conformation polymorphism analysis.
RESULTSSeveral
dominant bands were seen in all samples and some of the dominant bands
were common among the two or three separate regions of each synovial sample.
CONCLUSIONInfiltrated
B cells are oligoclonal, and an antigen driven immune response may play
a part in the progression of the disease process in OA.
© 2001 by Annals of the Rheumatic Diseases
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