Extended report
CpG-DNA derived from sera in systemic lupus erythematosus
enhances ICAM-1 expression on endothelial cells
M Miyata, O Ito, H Kobayashi, T Sasajima, H Ohira, S Suzuki, R Kasukawa
Department of
Internal Medicine II, Fukushima Medical University School of Medicine,
1 Hikarigaoka, Fukushima City, Fukushima 960-1295, Japan
Correspondence to: Dr Miyata metm{at}msg.biglobe.ne.jp
Accepted for publication 24 October
2000
OBJECTIVE
To examine
the effect of transfection of oligodeoxynucleotides (ODNs) containing a
CpG motif (CpG-ODN), of which the sequence was derived from circulating
DNA in the sera of patients with systemic lupus erythematosus (SLE), on
the expression of intercellular adhesion molecule-1 (ICAM-1) and
synthesis of mRNA for proinflammatory cytokines and ICAM-1 in human
umbilical vein endothelial cells (EC).
METHODSA CpG-ODN or a
control analogue, GpC-ODN, was transfected into EC. ICAM-1 expression
was examined by flow cytometry, and expression of mRNA in EC encoding
interleukin 1 (IL1), IL6, IL8, tumour necrosis factor 
(TNF),
interferon 
(IFN), and ICAM-1 was examined by semiquantitative
reverse transcriptase-polymerase chain reaction.
RESULTSThe CpG-ODN
augmented the expression of ICAM-1 on EC determined by flow cytometry
and increased mRNA levels of IL6, IL8, TNF, IFN, and ICAM-1, but
the GpC-ODN did not.
CONCLUSIONSynthesised
DNA, with a sequence corresponding to that of the fragment containing
the CpG motif, in sera of patients with SLE was found to enhance ICAM-1
expression on EC, suggesting the participation of circulating DNA
fragments in the pathogenesis of vasculitis in SLE.
© 2001 by Annals of the Rheumatic Diseases
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