Ann Rheum Dis 2001;60:116-123 ( February )

Extended report

Autoantibody profiles in the sera of European patients with myositis R Brouwer^a, G J D Hengstman^b, W Vree Egberts^a, H Ehrfeld^c, B Bozic^d, A Ghirardello^e, G Grøndal^f, M Hietarinta^g, D Isenberg^h, J R Kaldenⁱ, I Lundberg^f, H Moutsopoulos^j, P Roux-Lombard^k, J Vencovsky^l, A Wikman^f, H P Seelig^c, B G M van Engelen^b, W J van Venrooij^a

^a Department of Biochemistry, University of Nijmegen, Nijmegen, The Netherlands, ^b Neuromuscular Centre Nijmegen, Institute of Neurology, University Hospital Nijmegen, Nijmegen, The Netherlands, ^c Institute of Immunology and Molecular Genetics, Karlsruhe, Germany, ^d Department of Rheumatology, Ljubljana, Slovenia, ^e Division of Rheumatology, Padova, Italy, ^f Department of Rheumatology, Stockholm, Sweden, ^g Department of Medicine, Turku University, Finland, ^h Bloomsbury Rheumatology Unit, London, United Kingdom, ⁱ Institute of Clinical Immunology, Erlangen-Nürnberg, Germany, ^j Department of Pathophysiology, National University, Athens, Greece, ^k Division of Immunology and Allergy, University of Geneva, Switzerland, ^l Institute of Rheumatology, Prague, Czech Republic

Correspondence to: Dr W J van Venrooij, Department of Biochemistry, University of Nijmegen, PO Box 9101, NL-6500 HB, Nijmegen, The Netherlands w.vanvenrooij{at}bioch.kun.nl Accepted 22 August 2000

OBJECTIVETo determine the prevalence of myositis specific autoantibodies (MSAs) and several myositis associated autoantibodies (MAAs) in a large group of patients with myositis.
METHODSA total of 417 patients with myositis from 11 European countries (198 patients with polymyositis (PM), 181 with dermatomyositis (DM), and 38 with inclusion body myositis (IBM)) were serologically analysed by immunoblot, enzyme linked immunosorbent assay (ELISA) and/or immunoprecipitation.
RESULTSAutoantibodies were found in 232 sera (56%), including 157 samples (38%) which contained MSAs. The most commonly detected MSA was anti-Jo-1 (18%). Other anti-synthetase, anti-Mi-2, and anti-SRP autoantibodies were found in 3%, 14%, and 5% of the sera, respectively. A relatively high number of anti-Mi-2 positive PM sera were found (9% of PM sera). The most commonly detected MAA was anti-Ro52 (25%). Anti-PM/Scl-100, anti-PM/Scl-75, anti-Mas, anti-Ro60, anti-La, and anti-U1 snRNP autoantibodies were present in 6%, 3%, 2%, 4%, 5%, and 6% of the sera, respectively. Remarkable associations were noticed between anti-Ro52 and anti-Jo-1 autoantibodies and, in a few sera, also between anti-Jo-1 and anti-SRP or anti-Mi-2 autoantibodies.
CONCLUSIONSThe incidence of most of the tested autoantibody activities in this large group of European patients is in agreement with similar studies of Japanese and American patients. The relatively high number of PM sera with anti-Mi-2 reactivity may be explained by the use of multiple recombinant fragments spanning the complete antigen. Furthermore, our data show that some sera may contain more than one type of MSA and confirm the strong association of anti-Ro52 with anti-Jo-1 reactivity.

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© 2001 by Annals of the Rheumatic Diseases
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