Synovial membrane p53 protein immunoreactivity in rheumatoid arthritis patients

doi:10.1136/ard.59.2.143

Annals of the Rheumatic Diseases 2000;59:143-145; doi:10.1136/ard.59.2.143

Ann Rheum Dis 2000;59:143-145 ( February )

Concise report

Synovial membrane p53 protein immunoreactivity in rheumatoid arthritis patients C Soon Lee^b, Ian Portek^a, John Edmonds^a, Bruce Kirkham^a

^a Department of Rheumatology, St George Hospital, University of New South Wales, Australia, ^b Department of Anatomical Pathology, Royal Prince Alfred Hospital, University of Sydney, NSW, Australia

Correspondence to: Dr Kirkham and A/Professor Soon Lee, Department of Rheumatology, St George Hospital, Gray Street, Kogarah, New South Wales, Australia 2217

Accepted for publication 2 November 1999

OBJECTIVES $---$ To examine the expression of the p53 protein in synovial membrane of rheumatoid arthritis (RA) patients and to compare thiswith the expression in normal synovial tissues in subjects withoutRA.
METHODS $---$ Immunohistological expression of the p53 protein was studied using a streptavidin-biotin-peroxidase method and the monoclonalantibody DO-7, an antibody directed against both wild and mutantforms of p53 protein, in synovial tissues of RA patients (n=10)and from subjects with no known joint disease (n=4).
RESULTS $---$ p53 protein expression was present in a small percentage of synovial cells in the majority of the RA patients (n=8; 80%) andin half of the normal control cases with no inflammatory jointdisease (n=2; 50%). No sample had more than 5% cells stainingwith intranuclear pattern. The difference in synovial p53 immunoreactivitybetween the RA patients and normal controls is not statisticallysignificant (p= 0.64; $chi$ ² contingencytest).
CONCLUSIONS $---$ This study has shown that p53 protein is only weakly expressed in the rheumatoid synovial membrane, with a low percentageof p53 protein immunostaining cells present, with intranuclearstaining. These results suggest this is wild type p53 proteinrather than mutant protein. These findings suggest that synovialp53 protein expression may not be important in the pathogenesisof RA and may only represent a reactive repair process to DNAdamage secondary to the immune and inflammatory reactions associatedwith thedisease.

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