Apoptosis in normal and osteoarthritic human articular cartilage

doi:10.1136/ard.59.12.959

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Annals of the Rheumatic Diseases 2000;59:959-965; doi:10.1136/ard.59.12.959

Ann Rheum Dis 2000;59:959-965 ( December )

Extended report

Apoptosis in normal and osteoarthritic human articular cartilage F Héraud^a, A Héraud^c, M-F Harmand^a ^b

^a INSERM U443, Victor Segalen University, Bordeaux, France, ^b LEMI (Laboratoire d'Evaluation des Matériels Implantables), Technopole Montesquieu, Martillac, France, ^c Department of Medicine and Rheumatology, Robert Boulin Hospital, Libourne, France

Correspondence to: Dr M-F Harmand, INSERM U443, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux cedex, France u443{at}bordeaux.inserm.fr

Accepted for publication 26 April 2000

OBJECTIVES $---$ To investigate whether apoptosis occurs in osteoarthritis (OA), and if this phenomenon is modulated by human recombinant interleukin1 $beta$ (hrIL1 $beta$ ).
METHODS $---$ Human articular cartilage samples were obtained at the time of hip arthroplasty because of femoral neck fracture (normal cartilage)(n=4) or advanced coxarthrosis (OA cartilage) (n=14). Apoptoticchondrocytes, isolated by collagenase digestion and cultivatedfor 24 hours, or present in situ in frozen cartilage sections,were quantified by fluorescent microscopy using two apoptosismarkers: the TUNEL reaction, which detects nuclear DNA fragmentation,and Annexin-V-fluos, which labels at the membrane level the externalisationofphosphatidylserine.
RESULTS $---$ In OA cartilage 18-21% of chondrocytes showed apoptotic features, compared with 2-5% in normal cartilage. The results weresimilar for the two comparative studies (in situ and in vitro)and for both apoptosis markers. Moreover, hrIL1 $beta$ increased theapoptosis rate in vitro in a dose dependent manner in OA and normalchondrocytes.
CONCLUSION $---$ These results suggest that apoptosis may be an important factor in the evolution of OA and may be a new target for treatmentofOA.

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