Extended report
Tumour necrosis factor 
stimulated rheumatoid synovial
microvascular endothelial cells exhibit increased shear rate dependent
leucocyte adhesion in vitro
S E Abbota, W J D Whishb, C Jennisond, D R Blakec, C R Stevensc
a Departments of
Pharmacy and Pharmacology, b Biochemistry, c Postgraduate Medicine, d and
Mathematical Sciences, e University of Bath, Bath
Correspondence to: Dr S E Abbot, Department of Pharmacy and Pharmacology, University of Bath, Claverton Down, Bath, BA2 7AY.
Accepted for publication 4 May 1999
OBJECTIVE
To
investigate endothelial cell adhesion molecule expression and leucocyte
adhesion to endothelial cells isolated from the microvasculature of
rheumatoid arthritic synovial tissue (SMEC) in comparison with similar
cells isolated from healthy subcutaneous adipose tissue (ADMEC) or from
umbilical veins (HUVEC).
METHODSCultured
endothelial cells were treated with tumour necrosis factor 
(TNF)
for 2-24 hours before the assessment of cell surface E-selectin,
vascular (VCAM-1) or intercellular cell adhesion molecule-I (ICAM-1)
expression. Neutrophil and T lymphocyte adhesion to TNF treated
endothelial cells was assessed using static and shear dependent assay systems.
RESULTSVCAM-1
expression by SMEC was significantly less sensitive to TNF
stimulation than HUVEC or ADMEC. E-selectin expression by SMEC appeared
to be more sensitive to TNF stimulation and maximal expression was
about 30% greater in comparison with HUVEC or ADMEC. Sensitivity to
TNF induction and maximal ICAM-1 expression was similar in all three
endothelial cell types. Static neutrophil adhesion to TNF stimulated
SMEC was significantly increased in comparison with HUVEC, however this
phenomenon was dependent on the presence of neutralising antibodies to
ICAM-1. At shear rates in excess of 2.4 dynes/cm2
significantly more neutrophils and, predominantly CD45RO+, T lymphocytes adhered to TNF stimulated SMEC than HUVEC.
CONCLUSIONRheumatoid
synovial endothelial cells differentially regulate E-selectin and
VCAM-1. The increased ability of TNF stimulated synovial endothelial
cells to support leucocyte adhesion may help to explain the leucocyte,
in particular CD45RO+ T-lymphocyte, recruitment observed in the
rheumatoid synovium.
© 1999 by Annals of the Rheumatic Diseases
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
- Full Text
- Full Text (PDF)
- Submit a response
- Alert me when this article is cited
- Alert me when eLetters are posted
- Alert me if a correction is posted
- Citation Map
Services
- Email this link to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Add article to my folders
- Download to citation manager
- Request Permissions
Citing Articles
Google Scholar
PubMed
Topic Collections
Bookmark with
Register for free content
The full back archive is now available for all BMJ Journals. Institutional subscribers may access the entire archive as part of their subscription. Personal subscribers will also have access to all content when logged in. Non-subscribers who register have free access to all articles published before 2006 right back to volume 1 issue 1. Register here to access the free archive of all BMJ Journals.
Don't forget to sign up for content alerts so you keep up to date with all the articles as they are published.
