Extended report
Characterisation of T cell clonotypes that accumulated in
multiple joints of patients with rheumatoid arthritis
Manae Kurokawaa c, Tomohiro Katoa, Kayo Masuko-Hongoa, Shin-ichiro Uedad, Tetsuji Kobatab, Mitsuo Okuboe, Tomoe Nishimakic, Tatsuya Akazaf, Shin-ich Yoshinog, Reiji Kasukawac, Kusuki Nishiokaa, Kazuhiko Yamamotod
a Rheumatology,
Immunology and Genetic Program, Institute of Medical Science, St
Marianna University School of Medicine, Kawasaki, Japan, b Division of Immunology, Institute for Medical
Science, Dokkyo University School of Medicine, Shimotsuga,
Japan, c Department of Internal
Medicine II, Fukushima Medical College, Fukushima, Japan, d Division of Allergology and Rheumatology,
Department of Medicine, University of Tokyo, Bunkyo-ku, Tokyo, Japan, e Blood
Transfusion Service, Saitama Medical Center, Saitama Medical School,
Kawagoe, Japan, f Japanese Red
Cross Central Blood Centre, Shibuya-ku, Tokyo, Japan, g Department of Joint Disease and Rheumatism,
Nippon Medical School, Bunkyo-ku, Tokyo, Japan
Correspondence to: Dr M Kurokawa, Rheumatology, Immunology and Genetic Program, Institute of Medical Science, St Marianna University School of Medicine, 2-16-1, Sugao, Miyamae-ku, Kawasaki, 216-8512, Japan.
Accepted for publication 4 May 1999
OBJECTIVE
To
investigate whether identical T cell clonotypes accumulate in multiple
rheumatoid joints, the clonality of T cells that had infiltrated into
synovial tissue (ST) samples simultaneously obtained from multiple
joints of patients with rheumatoid arthritis (RA) was analysed.
METHODST cell
receptor (TCR) 
gene transcripts, amplified by reverse
transcription-polymerase chain reaction from ST and peripheral blood
lymphocytes of five RA patients, were subjected to single strand
conformation polymorphism analysis and DNA sequencing.
RESULTSApproximately
40% of accumulated T cell clonotypes found in one joint of a patient
were found in multiple joints in the same patient. Furthermore,
identical amino acid sequences were found in TCR junctional regions
of these clonotypes from different patients with at least one HLA
molecule match.
CONCLUSIONSThe T cell
clonotypes accumulating in multiple rheumatoid joints may be involved
in the perpetuation of polyarthritis by reacting to antigens common to
these multiple joints.
© 1999 by Annals of the Rheumatic Diseases
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