Concise reports
Chondroitin sulphation patterns in synovial fluid in
osteoarthritis subsets
Samantha Lewisa, Margot Crossmana, Joanne Flannellyc, Carolyn Belcherb, Michael Dohertyb, Michael T Baylissc, Roger M Masona
a Molecular
Pathology Section, Division of Biomedical Sciences, Imperial College
School of Medicine, South Kensington, London SW7 2AZ, b Rheumatology Unit, City Hospital, Nottingham, c Department
of Veterinary Basic Sciences, The Royal Veterinary College, London
Correspondence to: Professor R M Mason.
Accepted for publication 26 March 1999
OBJECTIVES
To
determine concentrations of chondroitin sulphate (CS) disaccharides in
knee synovial fluid (SF) from normal subjects and patients with
osteoarthritis (OA) or rheumatoid arthritis (RA), to test whether these
variables differ between different diseases and subsets of OA.
METHODSOA was
subdivided into large joint OA (LJOA), nodal generalised OA (NGOA), and
OA with calcium pyrophosphate crystal deposition (CPA), with 25, 9, and
11 people in each subset respectively. The SF of 13 normal subjects was
also volunteered for analysis along with 15 RA patients. Clinical
assessment of inflammation (0-6) was undertaken on OA and RA knees.
Concentrations of unsaturated CS disaccharides 
di6S and di4S were
measured by capillary zone electrophoresis.
RESULTSConcentrations
of di6S were lower in RA (5.90 ng/ml) and OA (13.24 ng/ml) fluids
compared with normal (21.0 ng/ml) but no significant differences were
seen between disease and normal fluids for di4S (about 4-6 ng/ml).
The ratio of di6S:di4S were RA<OA<normal subjects (p<0.001 for
all comparisons). The disaccharide concentration values along with the
ratios are below. Higher di6S:di4S ratios were obtained for LJOA
and CPA compared with NGOA. Uninflamed knees had lower concentrations
of di6S than inflamed knees (p<0.01). In patients with bilateral
samples, there were strong correlations between right and left knees
for all SF variables.
CONCLUSIONSAltered
ratios of CS sulphation patterns occur in OA and within OA subsets.
These further justify considering NGOA as a subset with a different aetiopathogenesis.
© 1999 by Annals of the Rheumatic Diseases
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