Extended reports
A quantitative method for detecting deposits of amyloid A protein
in aspirated fat tissue of patients with arthritis
Division of
Rheumatology, Department of Medicine, University Hospital, Groningen,
the Netherlands
Correspondence to: Dr B PC Hazenberg, Department of Rheumatology, University Hospital, PO Box 30.001, 9700 RB Groningen, the Netherlands.
Accepted for publication 23 October 1998
OBJECTIVE
To describe
a new, quantitative, and reproducible method for detecting deposits of
amyloid A protein in aspirated fat tissue and to compare it with smears
stained with Congo red.
METHODSAfter
extraction of at least 30 mg of abdominal fat tissue in guanidine, the
amyloid A protein concentration was measured by a monoclonal
antibody-based sandwich ELISA.
RESULTSThe
concentrations in 24 patients with arthritis and AA amyloidosis (median
236, range 1.1-8530 ng/mg tissue) were higher (p<0.001) than in
non-arthritic controls, uncomplicated rheumatoid arthritis, and other
types of systemic amyloidosis (median 1.1, range 1.1-11.6 ng/mg
tissue). Patients with extensive deposits, according to Congo red
staining, had higher concentrations than patients with minute deposits.
CONCLUSIONThis
is a new, quantitative, and reproducible method for detecting deposits
of amyloid A protein in aspirated fat tissue of patients with
arthritis, even when minute deposits are present as detected in smears
stained with Congo red.
© 1999 by Annals of the Rheumatic Diseases
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