Concise report
Expression of adhesion molecules on synovial fluid and peripheral
blood monocytes in patients with inflammatory joint disease and
osteoarthritis
M Köller, M Aringer, H Kiener, L Erlacher, K Machold, G Eberl, A Studnicka-Benke, W Graninger, J Smolen
Division of
Rheumatology, Department of Internal Medicine III, University of
Vienna, Währinger Gürtel 18-20, A-1090 Vienna, Austria
Correspondence to: Dr M Köller.
Accepted for publication 15 June 1999
OBJECTIVE
To determine
the presence of adhesion molecules on monocytes/macrophages (M
) from
peripheral blood (PB) and synovial fluid (SF) in patients with
osteoarthritis (OA) and inflammatory joint diseases (rheumatoid (RA)
and reactive arthritis (ReA)) in order to improve our understanding of
the possible mechanisms underlying the inflammatory process.
METHODSWhole blood
and SF cells were stained with monoclonal antibodies against CD11a
(LFA-1), CD15 s (sialyl-Lewis X), CD44, CD54, VLA-4, and HLA-DR
counterstained with anti-CD14 antibodies as a M marker for dual
fluorescence analysis by flowcytometry.
RESULTSOn PB-M,
CD15s was markedly increased in both RA as well as ReA compared with
OA. Furthermore, in the PB LFA-1, CD44, and HLA-DR showed a higher
surface density on M in ReA than in OA. Comparison between SF and PB
showed significantly higher CD44 and CD54 expression on SF-M. These
molecules play an important part in lymphocyte-M interaction.
CONCLUSIONIn PB from
patients with inflammatory joint diseases, M are activated, allowing
recruitment into the synovial compartment. These disorders, in contrast
with OA seem to be "systemic" in nature. Within the SF, different
adhesion molecules are expressed on CD14+ M as compared
with PB.
© 1999 by Annals of the Rheumatic Diseases
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