Extended report
Expression of laminins and their integrin receptors in different
conditions of synovial membrane and synovial membrane-like interface
tissue
Yrjö T Konttinena, Tian Fang Lic, Jing Wen Xub, Mickiaki Tagakif, Laura Piriläe, Timo Silvennoinend, Seppo Santavirtac, Ismo Virtanenb
a Department of
Medicine, Helsinki University Central Hospital, Helsinki,
Finland, b Department of
Anatomy, Institute of Biomedicine, University of Helsinki, Helsinki,
Finland, c Department of Orthopaedics and Traumatology,
Helsinki University Central Hospital, Helsinki, Finland, d Department of Surgery,
Helsinki City Hospital, Helsinki, Finland, e Department
of Medicine, Turku City Hospital, Turku, Finland, f Department of Orthopaedic Surgery, Yamagata
University School of Medicine, Yamagata, Japan
Correspondence to: Dr Y T Konttinen, Department of Anatomy, Institute of Biomedicine, PO Box 9 (Siltavuorenpenger 20 A), FIN-00014 University of Helsinki, Helsinki, Finland.
Accepted for publication 25 May 1999
OBJECTIVE
To
demonstrate the expression of laminins (Lns) and their integrin (Int)
receptors in different synovial samples and synovial membrane-like
interface tissues from well fixed and aseptically loosened total hip
replacement (THR), and the potential role of Ln-Int interaction in the
production of collagenases and cytokines.
METHODSImmunohistochemical
staining was done to detect the distribution of EHS Ln, Ln 
2, 3,
5, 
1, 2 chains and Int 1, 2, 3, 6, 1, 4
subunits in different samples. Double immunofluorescence labelling was
used to find colocalisation of Int 6 subunit and collagenase-1/collagenase-3/TNF/IL6.
RESULTSGeneral Ln
immunoreactivity was detected in all specimens. Ln 5, 1 and 2,
but not 2 and 3 chains were seen in the synovial lining and the
basement membrane of blood vessels with the intensity/extent of
labelling in the following rank order: rheumatoid arthritis (RA)
loosened prostheses, osteoarthritis, well fixed prostheses, traumatic
knees. Among Int subunits, staining for 1 was usually the strongest,
followed by staining for Int 6, 1, 3, and 2 subunits, with
the same rank order for overall expression of Lns. Int 4 subunit was
not detectable in most of the specimens. Double labelling focused on
Int 6 subunit disclosed its frequent colocalisation with
collagenases 1 and 3 and with tumour necrosis factor and interleukin 6 in synovial lining.
CONCLUSIONSynovial
lining contains Ln-10, Ln-11, and Int 61 and 11 receptors.
In aseptic loosening of THR, interface tissue has a similar Ln subtype
and Int receptor composition as RA synovium, which confirms its
"lining-like" phenotype. Synovial lining does not contain Ln-5
(33
2) or Int 64, which are components of epithelial
hemidesmosomes. The expression of Lns and their Int receptors is
upregulated in inflammation. The close spatial relation between Ln and
its Int receptors in synovial lining cells containing proteinases and
cytokines suggests a potential role in joint destruction and prosthetic loosening.
© 1999 by Annals of the Rheumatic Diseases
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