Extended report
Presentation of autoantibody to proliferating cell nuclear
antigen in patients with chronic hepatitis B and C virus infection
Bor-Show Tzangb, Tzy-Yen Chena, Tsai-Ching Hsua, Yin-Chang Liub, Gregory J Tsaya
a Department of
Medicine, Chung Shan Medical and Dental College, Taichung, Taiwan, b Department of Life Science, National Tsing-Hua
University, Hsin-Chu, Taiwan
Correspondence to: Dr G J Tsay, Department of Medicine, Chung Shan Medical and Dental College, 110 Section 1, Chien Kuo N Road, Taichung 402, Taiwan.
Accepted for publication 28 May 1999
OBJECTIVES
To study
the association of antibodies to proliferating cell nuclear antigen
(PCNA) in patients with chronic hepatitis B (HBV) and C (HCV) virus infection.
METHODSSera from 243 patients with chronic HBV infection; 379 patients with chronic HCV
infection; 80 patients with systemic lupus erythematosus (SLE); 28 patients with rheumatoid arthritis; 15 patients with Sjogren's
syndrome; eight with polymyositis; eight with primary biliary
cirrhosis; and 33 healthy control subjects were tested for the
presentation of anti-PCNA antibodies by enzyme linked immunosorbent
assay (ELISA) and immunoblotting using recombinant PCNA as antigen. The
distribution of immunoglobulin isotypes of anti-PCNA antibody was
measured by ELISA assay.
RESULTSBy ELISA,
anti-PCNA antibodies were detected in 30 (12.3%) patients with chronic
HBV infection, 71 (18.7%) patients with chronic HCV infection, and
five (6.3%) patients with SLE. The inhibition of binding with these
sera by purified PCNA was shown to exceed 71%. By immunoblotting, the
frequency of anti-PCNA in patients with chronic HBV and HCV infection
was 17 of 243 (7%) and 41 of 379 (11%), respectively. Absorption
studies on indirect immunofluorescence showed the typical nuclear
speckled staining pattern by anti-PCNA sera was abolished by
preincubation of sera with PCNA. Anti-PCNA antibody was not detected in
sera from patients with autoimmune diseases except SLE. Anti-PCNA
antibodies in patients with chronic HBV and HCV infection were
predominantly IgG.
CONCLUSIONThese data
suggest that anti-PCNA antibody are also present in patients with
chronic HBV and HCV infection. Anti-PCNA antibody may not be specific
for SLE.
© 1999 by Annals of the Rheumatic Diseases
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