Extended reports
Antigen specificity of antihistone antibodies in systemic
sclerosis
a Department of
Dermatology, Kanazawa University School of Medicine, Kanazawa, Japan, b Department of Dermatology, Faculty of Medicine,
University of Tokyo, Tokyo, Japan
Correspondence to: Dr S Sato, Department of Dermatology, Kanazawa University School of Medicine, 13-1, Takara-machi, Kanazawa 920-8641, Japan.
Accepted for publication 15 June 1998
OBJECTIVES
The aim of
the study was to determine the prevalence and clinical significance of
antibodies to individual histone components in systemic sclerosis (SSc).
METHODSSerum samples
from patients with limited cutaneous SSc (lSSc; n=42) and diffuse
cutaneous SSc (dSSc; n=28) were examined for IgG and/or IgM antibodies
to individual histone components and complexes by enzyme linked
immunosorbent assay (ELISA).
RESULTSThe
level of IgG antibody to total histones was significantly higher in
lSSc and dSSc than in normal controls. The level of IgM antibody to
total histones was significantly higher in lSSc, but not in dSSc, than
in normal controls. IgG antibody to total histones tended to be
increased in dSSc when compared with that in lSSc. On the other hand,
IgM antibody to total histones tended to be increased in lSSc when
compared with that in dSSc. Although SSc showed various antihistone
specificities, H2B, H2A-H2B, (H2A-H2B)-dsDNA were main antigens
recognised by IgG antibodies in both lSSc and dSSc. Although IgM
antibodies to H2B and H2A-H2B were also detected in both lSSc and dSSc,
serum samples from lSSc patients exhibited highest IgM reactivity with H1.
CONCLUSIONSSc may be
included among conditions in which heterogeneous antihistone antibodies
are produced. IgM antibodies to the most accessible histone H1 may be
related to mild clinical features (lSSc) and IgG antibodies to the
inner core molecules of native histone such as H2B or complexes
including H2B may be associated with severe clinical features (dSSc) in Ssc.
© 1998 by Annals of the Rheumatic Diseases
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