Extended reports
Iron, lactoferrin and iron regulatory protein activity in the
synovium; relative importance of iron loading and the inflammatory
response
a Department of Immunology, Western Infirmary, Glasgow,
G11 6NT, b Centre for Rheumatic Diseases, Royal Infirmary, Glasgow
Correspondence to: Dr J H Brock.
Accepted for publication 10 March
1998
OBJECTIVES
To determine the ability of lactoferrin
in rheumatoid arthritis (RA) synovial fluid to bind "free" iron,
and to study the regulatory mechanisms therein that control iron homeostasis.
METHODS"Free" iron was determined by the
bleomycin assay and lactoferrin concentrations by enzyme linked
immunosorbent assay. The activities of iron regulatory protein (IRP)
and NF-
B in synovial fluid cells were assayed by mobility shift assay.
RESULTS30% of synovial fluids contained
"free" iron and in these, lactoferrin concentrations were
significantly lower than in those with no "free" iron (p<0.01).
Addition of exogenous lactoferrin consistently reduced the amount of
"free" iron in positive synovial fluids. IRP activity in synovial
cells did not correlate with synovial fluid iron concentrations but did
correlate with NF-B activation and with serum C reactive protein.
CONCLUSIONLactoferrin may prevent iron mediated
tissue damage in RA by reducing "free" synovial iron concentration
when inflammatory stimuli have disregulated IRP mediated iron homeostasis.
© 1998 by Annals of the Rheumatic Diseases
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