Expression of alpha  and beta  subunits of the integrin superfamily in articular cartilage from macroscopically normal and osteoarthritic human femoral heads

doi:10.1136/ard.57.5.303

Annals of the Rheumatic Diseases 1998;57:303-308; doi:10.1136/ard.57.5.303

Ann Rheum Dis 1998;57:303-308 ( May )

Extended reports

Expression of $alpha$ and $beta$ subunits of the integrin superfamily in articular cartilage from macroscopically normal and osteoarthritic human femoral heads Keld Ostergaard,^a Donald M Salter,^b Jorgen Petersen,^a Klaus Bendtzen,^a Jesper Hvolris,^c Claus B Andersen^d

^a Institute for Inflammation Research (IIR), RHIMA-Centre, National University Hospital/Rigshospitalet, Copenhagen, Denmark, ^b Department of Pathology, University of Edinburgh, Medical School, Edinburgh, United Kingdom, ^c Department of Orthopaedics, Hvidovre University Hospital, Hvidovre, Denmark, ^d Department of Pathology, National University Hospital/Rigshospitalet, Copenhagen, Denmark

Correspondence to: Dr K Ostergaard, Institute for Inflammation Research (IIR), 7541, RHIMA-Centre, National University Hospital/Rigshospitalet, Tagensvej 22, DK-2200 Copenhagen N, Denmark.

Accepted for publication 11 March 1998

OBJECTIVE $---$ The objective of this study was to detail the topographical and zonal distribution of $alpha$ and $beta$ subunits of the integrin superfamilyin normal and osteoarthriticcartilage.
METHODS $---$ Immunohistochemistry utilising antibodies towards $alpha$ and $beta$ subunits was performed on cryostat sections of human articular cartilagefrom macroscopically normal (n = 6) and osteoarthritic (n = 6)femoral heads. Samples of articular cartilage were obtained from12 topographically distinct sites from each femoral head. Eachsection was divided into zones (superficial, middle, deep) andstaining scores wererecorded.
RESULTS $---$ Normal cartilage stained for integrin subunits $alpha$ 1, $alpha$ 5, $alpha$ V, $beta$ 1, $beta$ 4, and $beta$ 5, but not for $alpha$ 2, $alpha$ 3, $alpha$ 4, $alpha$ 6, $beta$ 2, $beta$ 3, and $beta$ 6. Intactand non-intact residual cartilage from osteoarthritic femoralheads stained for $alpha$ 1, $alpha$ 2, $alpha$ 5, $alpha$ V, $beta$ 1, $beta$ 4, and $beta$ 5. Staining wasoccasionally seen for $alpha$ 4 and $beta$ 2, but not for $alpha$ 3, $alpha$ 6, $beta$ 3, and $beta$ 6.There was no topographical variation in the staining for any ofthe subunits in either normal or osteoarthritic cartilage. Theonly subunit that displayed a zonal variation was $alpha$ V; stainingfor this subunit was most pronounced in the superficial zone comparedwith the middle and deepzones.
CONCLUSION $---$ Chondrocytes in normal and osteoarthritic cartilage express the integrin subunits $alpha$ 1, $alpha$ 5, $alpha$ V, $beta$ 1, $beta$ 4, and $beta$ 5. Chondrocytesin osteoarthritic cartilage, in addition, express the $alpha$ 2, $alpha$ 4,and $beta$ 2 subunits. The $alpha$ v subunit is expressed by more chondrocytesin the superficial zone in comparison with cells in the deeperzones. None of the subunits display topographical variation inexpression.

Keywords: cartilage; integrins; immunohistochemistry; osteoarthritis

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