Extended reports
Hydrostatic pressure induces expression of interleukin 6 and
tumour necrosis factor 
mRNAs in a chondrocyte-like cell line
a Department of Orthopaedic Surgery, Kyoto Prefectural
University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto
602-0841, Japan, b Department of Laboratory and Molecular
Medicine, Kagoshima University School of Medicine, Kagoshima Japan, c Department of Biochemistry and Molecular
Dentistry, Okayama University Dental School, Okayama, Japan, d Department of Microbiology, Kyoto
Prefectural University of Medicine, Kyoto Japan
Correspondence to: Dr Kubo.
Accepted for publication 27 February 1998
OBJECTIVE
To clarify the effect of pressure on the
expressions of proteoglycan core protein and metabolism related
cytokines in a chondrocyte-like cell line, HCS-2/8.
METHODSHCS-2/8 cells were exposed to 1, 5, 10, or
50 MPa of hydrostatic pressure (HP) for two hours, and mRNA expressions
of interleukin 6 (IL6) and tumour necrosis factor 
(TNF) were
examined by using reverse transcription-polymerase chain reaction
(RT-PCR) method with specific primer sets; and mRNA of proteoglycan
core protein, stromelysin, and tissue inhibitor of metalloproteinase 1 (TIMP1) were measured with northern blotting.
RESULTSHP exposure caused temporal morphological
changes of the cells, but did not affect cellular viability. IL6 and
TNF mRNA expressions were not observed in the control cells under
the atmospheric pressure, whereas in the cells treated with HP,
pressure dependent enhancement of IL6 mRNA expression was observed
between 30 minutes and four hours after the HP release. TNF mRNA
expression also increased 30 minutes after the exposure to 50 MPa of HP
and disappeared four hours later. Proteoglycan core protein mRNA levels
increased between 30 minutes and four hours after the exposure to 1 or
5 MPa of HP, whereas the levels decreased after 10 or 50 MPa of HP.
Stromelysin and TIMP1 mRNA signals did not respond to HP.
CONCLUSIONSHP at excessively high levels induced
IL6 and TNF expression and reduced the expression of proteoglycan
core protein, while physiological levels of HP increased the expression
of proteoglycan core protein. These findings are important when
considering the pathology of osteoarthritis.
;
interleukin 6;
mRNA
© 1998 by Annals of the Rheumatic Diseases
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