Concise reports
Down regulation by iron of prostaglandin E2
production by human synovial fibroblasts
Second Department of
Internal Medicine, Kochi Medical School, Nankoku City 783-8505, Japan
Correspondence to: Dr K Nishiya.
Accepted for publication 23 September 1998
OBJECTIVE
To examine the effect of iron on the
prostaglandin (PG) E2 production by human synovial
fibroblasts in vitro.
METHODSHuman synovial fibroblasts were isolated
from synovial tissue of rheumatoid arthritis (RA) and osteoarthritis
(OA) patients and cultured in medium. Synovial fibroblasts were
stimulated by human recombinant interleukin (IL) 1
(0.1-10 ng/ml)
with or without ferric citrate (Fe-citrate, 0.01-1 mM). The amount of
PGE2 in the culture medium was measured by an enzyme linked
immunosorbent assay.
RESULTSThe production of PGE2 by the
synovial fibroblasts was increased by stimulation with IL1 at all
concentrations tested. Fe-citrate but not sodium citrate (Na-citrate)
down regulated the production of PGE2 by the synovial
fibroblasts, both with and without stimulation by IL1. Fe-citrate
inhibited the spontaneous PGE2 production by the cells in a
dose dependent manner, and a maximum inhibition by Fe-citrate was
observed at the concentration of 0.1 mM with IL1 stimulation. The
down regulation by iron was reversed by the co-addition of
desferrioxamine (100 µg/ml), an iron chelator.
CONCLUSIONIron down regulates the
PGE2 production by synovial fibroblasts in vitro.
© 1998 by Annals of the Rheumatic Diseases
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