Extended reports
Bisphosphonates in bone cement inhibit PMMA particle induced bone
resorption
Nuffield Orthopaedic Centre, University of
Oxford, Oxford
Correspondence to: Dr N A Athanasou, Department of Pathology, Nuffield Orthopaedic Centre, Headington, Oxford, OX3 7LD.
Accepted for publication 29 July 1998
OBJECTIVE
Wear particle induced bone resorption is
thought to be one of the mechanisms that contribute to implant
loosening. It has previously been shown that macrophages, in response
to polymethylmethacrylate (PMMA) particles, differentiate into bone
resorbing osteoclasts, and that this process is inhibited by a
bisphosphonate, etidronate (EHDP). The aim of this study was to
determine whether incorporating EHDP in bone cement could reduce PMMA
associated bone resorption.
METHODSTwo concentrations of EHDP were mixed with
PMMA monomer before polymerisation. Particles of PMMA (1-10 µm) were
generated then added to mouse monocytes cocultured with UMR106 rat
osteoblast-like cells and the extent of osteoclast differentiation was
determined by assessing the extent of tartrate resistant acid
phosphatase (TRAP) staining and measuring the amount of lacunar bone resorption.
RESULTSThe addition of PMMA to
monocyte-UMR106 cocultures resulted in a marked increase in the number
of TRAP positive osteoclast-like cells and a significant increase in
the number of lacunar resorption pits compared with control cultures to
which no particles had been added. After the addition of particles of
PMMA + 20 mg EHDP, significantly fewer lacunar pits (p=0.00006) and
fewer TRAP positive cells were noted compared with cocultures
containing PMMA particles alone.
CONCLUSIONSThese results indicate that by
mixing a bisphosphonate with bone cement, it is possible to inhibit
PMMA particle induced bone resorption. This bisphosphonate inhibition
of PMMA biomaterial wear particle containing macrophage-osteoclast
differentiation and bone resorption may provide a possible therapeutic
strategy to prevent or to control the osteolysis of aseptic loosening.
© 1998 by Annals of the Rheumatic Diseases
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
-
Peter, B., Zambelli, P.-Y., Guicheux, J., Pioletti, D. P.
(2005). The effect of bisphosphonates and titanium particles on osteoblasts: AN IN VITRO STUDY. J Bone Joint Surg Br
87-B: 1157-1163
[Abstract] [Full Text] -
Bhandari, M., Bajammal, S., Guyatt, G. H., Griffith, L., Busse, J. W., Schunemann, H., Einhorn, T. A.
(2005). Effect of Bisphosphonates on Periprosthetic Bone Mineral Density After Total Joint Arthroplasty. A Meta-Analysis. JBJS
87: 293-301
[Abstract] [Full Text] -
Millett, P. J., Allen, M. J., Bostrom, M. P.G.
(2002). Effects of Alendronate on Particle-Induced Osteolysis in a Rat Model. JBJS
84: 236-249
[Abstract] [Full Text] -
Sakai, H, Jingushi, S, Shuto, T, Urabe, K, Ikenoue, T, Okazaki, K, Kukita, T, Kukita, A, Iwamoto, Y
(2002). Fibroblasts from the inner granulation tissue of the pseudocapsule in hips at revision arthroplasty induce osteoclast differentiation, as do stromal cells. Ann Rheum Dis
61: 103-109
[Abstract] [Full Text]
- Full Text
- Full Text (PDF)
- Submit a response
- Alert me when this article is cited
- Alert me when eLetters are posted
- Alert me if a correction is posted
- Citation Map
Services
- Email this link to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Add article to my folders
- Download to citation manager
- Request Permissions
Citing Articles
Google Scholar
PubMed
Topic Collections
Bookmark with
Register for free content
The full back archive is now available for all BMJ Journals. Institutional subscribers may access the entire archive as part of their subscription. Personal subscribers will also have access to all content when logged in. Non-subscribers who register have free access to all articles published before 2006 right back to volume 1 issue 1. Register here to access the free archive of all BMJ Journals.
Don't forget to sign up for content alerts so you keep up to date with all the articles as they are published.
