Extended reports
Hyaluronan production in human rheumatoid fibroblastic synovial
lining cells is increased by interleukin 1
but inhibited by
transforming growth factor 1
Internal
Medicine I, National Defence Medical College, Saitama, Japan
Correspondence to: Dr M Kawakami, Internal Medicine I, National Defence Medical College Namiki 3-2, Tokorozawa, Saitama, 359-8513, Japan.
Accepted for publication 29 July 1998
OBJECTIVES
To investigate the regulatory roles of
interleukin 1
(IL1), tumour necrosis factor 
(TNF),
interferon 
(IFN) or transforming growth factor 1 (TGF1) on
hyaluronan (HA) synthesis by human fibroblastic synovial lining cells.
METHODSConcentrations of HA in culture
supernatants of fibroblastic synovial lining cell line (RAMAK-1 cell
line) with or without stimulation by IL1, TNF, IFN or TGF1
were measured by sandwich binding protein assay. Levels of HA synthase
mRNA of the cells with or without stimulation were detected by reverse
transcribed polymerase chain reaction. Molecular weights of HA in the
culture supernatants of the cells with or without stimulation were
measured using high performance gel permeation liquid chromatography.
RESULTSHA synthesis by the cells was not
significantly augmented by TNF or by IFN. It was significantly
stimulated by IL1 but inhibited by TGF1. Molecular weights of HA
in the culture supernatants of the cells were unchanged by stimulation
with TNF. They were remarkably increased by stimulation with IL1
and IFN, but reduced with TGF1.
CONCLUSIONIL1 is an up regulator of HA
synthesis, while TGF1 is a down regulator. HA production in the
synovial lining cells of inflamed joints (for example, rheumatoid
arthritis) might be regulated by the balance of these cytokines.
;
transforming growth factor 1
© 1998 by Annals of the Rheumatic Diseases
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