Extended reports
Analysis of LMP and TAP polymorphisms by polymerase chain
reaction-restriction fragment length polymorphism in rheumatoid
arthritis
a Instituto de Parasitología y Biomedicina
"López Neyra", CSIC, Granada, Spain, b Sección de Biología Teórica, Hospital Virgen de las
Nieves, Granada, Spain, c Servicio de
Reumatología, Hospital Virgen de las Nieves, Granada, Spain
Correspondence to: Dr J Martín, Instituto de Parasitología y Biomedicina "Lopez-Neyra", C/ Ventanilla n° 11 18001 Granada, Spain.
Accepted for publication 22 September 1997
OBJECTIVE
The aim of this study was to investigate
the relation between the polymorphism of large molecular weight
proteasome (LMP) (LMP2-LMP7) and transporter associated with antigen
processing (TAP) (TAP1-TAP2) genes and rheumatoid arthritis (RA).
METHODSSixty RA patients and 102 ethnically
matched unrelated healthy subjects were typed for LMP, TAP, and disease
associated HLA-DRB1 alleles by using a new strategy based on polymerase
chain reaction-restriction fragment length polymorphism (PCR-RFLP) with
amplification created restriction sites.
RESULTSThe polymorphism of LMP (LMP2-LMP7) and
TAP (TAP1-TAP2) genes was examined in shared epitope positive and
negative RA patients and controls. No significant differences in the
LMP or TAP allele frequencies were observed between the total patient
and control groups or the patients and controls positive or negative
for the shared epitope.
CONCLUSIONThe data suggest that the polymorphisms
of LMP and TAP genes do not have an important influence in the
pathogenesis of RA, although larger studies will be needed to provide
more conclusive evidence on the role of these genes in RA. A new,
highly reliable strategy for typing LMP alleles is also described.
© 1998 by Annals of the Rheumatic Diseases
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