Chondroitin and keratan sulphate epitopes, glycosaminoglycans, and hyaluronan in progressive versus non-progressive osteoarthritis

doi:10.1136/ard.56.2.119

Annals of the Rheumatic Diseases 1997;56:119-122; doi:10.1136/ard.56.2.119

Ann Rheum Dis 1997;56:119-122 ( February )

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Chondroitin and keratan sulphate epitopes, glycosaminoglycans, and hyaluronan in progressive versus non-progressive osteoarthritis Fiona Fawthrop,^a Reehana Yaqub,^a Carolyn Belcher,^a Michael Bayliss,^b Joanna Ledingham,^a Michael Doherty^a

^a Rheumatology Unit, City Hospital, Hucknall Road, Nottingham NG5 1PB, United Kingdom , ^b Biochemistry Division, Kennedy Institute of Rheumatology, 6 Bute Gardens, Hammersmith, London W6 7DW, United Kingdom

Correspondence to: Professor M Doherty.

Accepted for publication 28 October 1996

OBJECTIVE $---$ To determine if a single time point estimation of chondroitin sulphate (CS) or keratan sulphate (KS) epitopes, hyaluronan(HA), or total glycosaminoglycans (GAG) in knee synovial fluidat time of hospital referral can predict subsequent radiographicprogression of kneeosteoarthritis.
METHODS $---$ Two groups of hospital referred patients with knee osteoarthritis were compared: (1) a "progressive" group (n = 45), showingfurther reduction in radiographic joint space of at least onegrade (0-3) in at least one compartment; and (2) a "non-progressive"group (n = 25) in whom radiographs showed no change during themean follow up period of 2.3 years (median 2, range 1 to 5 years).Knee synovial fluid obtained at the first visit was examined byELISA for: CS epitopes, using monoclonal antibodies 3B3 and 7D4;KS epitope, using monoclonal antibody 5D4; and HA, using biotinylatedHA binding region of cartilage proteoglycan. Total sulphated GAGwere measured by dye binding with 1:9 dimethylmethyleneblue.
RESULTS $---$ In patients with bilateral synovial fluid data right and left knee values were closely correlated for all variables. Therewere no significant differences between CS and KS epitopes, HA,total sulphated GAG, or ratios of individual CS or KS epitopesto total GAG, between progressive and non-progressivegroups.
CONCLUSIONS $---$ Single time point estimation of CS, KS, HA, or total GAG in synovial fluid does not distinguish radiographically progressiveand non-progressive knee osteoarthritis patients followed fortwoyears.

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