Extended reports
Long term persistent accumulation of CD8+ T cells in synovial
fluid of rheumatoid arthritis
a Rheumatology, Immunology and Genetics Programme,
Institute of Medical Science, St Marianna University, Kawasaki, Japan
, b Medical
Institute of Bioregulation, Kyushu University, Oita, Japan
Correspondence to: Dr K Masuko-Hongo, Rheumatology, Immunology and Genetics Programme, Institute of Medical Science, St Marianna University, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa 216, Japan.
Accepted for publication 21 July 1997
OBJECTIVE
To characterise the type and kinetics
of T cell clones in synovial lesions of patients with rheumatoid
arthritis (RA).
METHODSMononuclear cells from serial samples of
synovial fluid (SF) and peripheral blood from nine RA patients were
separated phenotypically using antibody coated magnetic beads. After
mRNA preparation, reverse transcription-polymerase chain reaction
(RT-PCR) was performed to amplify V-D(N)-J (that is, the third
complementarity determining, CDR3) regions of their T cell receptor
beta chain genes. This was followed by single strand conformation
polymorphism (SSCP) analysis to detect the clonotypes of accumulating T
cells. Amino acid sequences of the dominant clones were also determined.
RESULTSAlthough peripheral T cells were
heterogeneous, accumulation of oligoclonal T cells was detected in SF.
The predominant accumulating clone was the CD8 subset, which was
persistently present in serial samples obtained over almost one year of
follow up. A proportion of these cells expressed CD25 or CD45RO, or
both, suggesting they are `memory' clones.
CONCLUSIONThe persistent presence of CD8+ T
cell clones in RA joints indicates that they may be involved in the
perpetuation of the chronic inflammatory process in RA joints.
© 1997 by Annals of the Rheumatic Diseases
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