Ann Rheum Dis 1997;56:45-51 ( January )

Extended reports

Increased expression of integrins on fibroblast-like synoviocytes from rheumatoid arthritis in vitro correlates with enhanced binding to extracellular matrix proteins N Rinaldi,^a M Schwarz-Eywill,^d D Weis,^b P Leppelmann-Jansen,^a M Lukoschek,^c U Keilholz,^a T F E Barth^b

^a University of Heidelberg, Heidelberg, Germany: Department of Internal Medicine V , ^b Institute of Pathology , ^c Department of Orthopaedic Surgery , ^d University of Dresden, Dresden, Germany: Department of Internal Medicine I

Correspondence to: Dr N Rinaldi, Department of Internal Medicine V, Hospitalstrasse, D-69115 Heidelberg, Germany.

Accepted for publication 24 October 1996

OBJECTIVETo compare in vitro expression of 1, 3, and 4 integrins in normal fibroblast-like synoviocytes (FBS) and in FBS from rheumatoid arthritis (RA) synovium and to investigate the adhesion of normal FBS and RA-FBS to the integrin binding extracellular matrix (ECM) proteins: collagen type IV, fibronectin, laminin, and tenascin.
METHODSExpression of integrin receptors of cultured FBS was detected by flow cytometry. Attachment of FBS to ECM proteins was quantified by adhesion assays. Inhibition studies were performed using monoclonal antibodies to the integrin subunits.
RESULTSCompared with normal FBS, RA-FBS showed increased expression of 1 to 6, 1, and 4 integrin subunits and enhanced binding of ECM proteins. Binding to ECM proteins was partly or completely blocked by an anti-1 integrin antibody and antibodies to 3, 5, and 6 integrin subunits. The blocking efficiency was significantly (P < 0.05) higher in RA-FBS than in normal FBS.
CONCLUSIONSThe enhanced expression of the 1 integrin receptors on cultured RA-FBS correlated with increased attachment to ECM proteins. Adhesion of normal and RA-FBS to ECM proteins is mediated through 1 integrin receptors. Therefore, the tight binding of rheumatoid FBS to the matrix via 1 integrins might play a role in ECM remodelling in the rheumatoid process in vivo.

---

© 1997 by Annals of the Rheumatic Diseases
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Hoberg, M., Rudert, M., Pap, T., Klein, G., Gay, S., Aicher, W. K (2007). Attachment to laminin-111 facilitates transforming growth factor {beta}-induced expression of matrix metalloproteinase-3 in synovial fibroblasts. Ann Rheum Dis 66: 446-451 [Abstract] [Full Text]  
• Kitagawa, A, Miura, Y, Saura, R, Mitani, M, Ishikawa, H, Hashiramoto, A, Yoshiya, S, Shiozawa, S, Kurosaka, M (2006). Anchorage on fibronectin via VLA-5 ({alpha}5{beta}1 integrin) protects rheumatoid synovial cells from Fas-induced apoptosis. Ann Rheum Dis 65: 721-727 [Abstract] [Full Text]  
• Huber, L. C., Distler, O., Tarner, I., Gay, R. E., Gay, S., Pap, T. (2006). Synovial fibroblasts: key players in rheumatoid arthritis. Rheumatology (Oxford) 45: 669-675 [Abstract] [Full Text]  
• Neidhart, M, Zaucke, F, von Knoch, R, Jungel, A, Michel, B A, Gay, R E, Gay, S (2005). Galectin-3 is induced in rheumatoid arthritis synovial fibroblasts after adhesion to cartilage oligomeric matrix protein. Ann Rheum Dis 64: 419-424 [Abstract] [Full Text]  
• Schedel, J, Distler, O, Woenckhaus, M, Gay, R E, Simmen, B, Michel, B A, Muller-Ladner, U, Gay, S (2004). Discrepancy between mRNA and protein expression of tumour suppressor maspin in synovial tissue may contribute to synovial hyperplasia in rheumatoid arthritis. Ann Rheum Dis 63: 1205-1211 [Abstract] [Full Text]  
• Wunder, A., Muller-Ladner, U., Stelzer, E. H. K., Funk, J., Neumann, E., Stehle, G., Pap, T., Sinn, H., Gay, S., Fiehn, C. (2003). Albumin-Based Drug Delivery as Novel Therapeutic Approach for Rheumatoid Arthritis. J. Immunol. 170: 4793-4801 [Abstract] [Full Text]  
• Rinaldi, N, Barth, T F E, Weis, D, Schwarz-Eywill, M, Pezzutto, A, Lukoschek, M, Brocai, D, Brado, B (1998). Loss of laminin and of the laminin receptor integrin subunit alpha 6 in situ correlates with cytokine induced down regulation of alpha 6 on fibroblast-like synoviocytes from rheumatoid arthritis. Ann Rheum Dis 57: 559-565 [Abstract] [Full Text]  
• Rinaldi, N., Weis, D, Brado, B, Schwarz-Eywill, M, Lukoschek, M, Pezzutto, A, Keilholz, U, Barth, T F E (1997). Differential expression and functional behaviour of the alpha v and beta 3 integrin subunits in cytokine stimulated fibroblast-like cells derived from synovial tissue of rheumatoid arthritis and osteoarthritis in vitro. Ann Rheum Dis 56: 729-736 [Abstract] [Full Text]