Increase of HLA-DRB1*0408 and -DQB1*0301 in HLA-B27 positive reactive arthritis

doi:10.1136/ard.56.1.37

Annals of the Rheumatic Diseases 1997;56:37-40; doi:10.1136/ard.56.1.37

Ann Rheum Dis 1997;56:37-40 ( January )

Extended reports

Increase of HLA-DRB1*0408 and -DQB1*0301 in HLA-B27 positive reactive arthritis Juha Tuokko,^a Helena Reijonen,^b Jorma Ilonen,^b Kaisa Anttila,^b Simo Nikkari,^a Timo Möttönen,^c Urpo Yli-Kerttula,^d Auli Toivanen^c

^a Turku University, Turku, Finland: Turku Immunology Centre and Department of Medical Microbiology , ^b Department of Virology , ^c Turku University Central Hospital, Turku, Finland: Division of Rheumatology, Department of Medicine , ^d Tampere University Hospital, Tampere, Finland: Division of Rheumatology, Department of Medicine

Correspondence to: Juha Tuokko MD, Department of Medical Microbiology, Turku University, Kiinamyllynkatu 13, FIN-20520, Turku, Finland.

Accepted for publication 21 October 1996

OBJECTIVE $---$ To study HLA class II association in reactivearthritis.
METHODS $---$ 63 patients with reactive arth-ritis and 46 with rheumatoid arthritis were included in the study. HLA-DR alleles were determinedby using a sequence specific PCR method. Oligonucleotide hybridisationwas used for definition of DRB1*04 subtypes and DQB1 alleles.HLA-B27 was determined by standard microcytotoxity test or byPCR. HLA-B27 subtyping was made bysequencing.
RESULTS $---$ 46 (73%) of 63 patients with reactive arthritis were HLA-B27 positive and 24 (38%) were HLA-DRB1*04 positive. When haplotypeswere inferred according to the known associations between DRB1and DQB1 alleles, the frequency of DRB1*04-DQB1*0301 haplotypewas found to be 13% (12/92) in HLA-B27 positive reactive arthritispatients, in contrast to 0% in HLA-B27 negative reactive arthritis(P = 0.04) and 1% in random controls (P = 0.0009). However, thiscombination was also found in 5% of 84 HLA-B27 positive controlhaplotypes, showing a linkage disequilibrium between B27 and thisparticular class II haplotype. HLA-DRB1*0408 subtype was foundin 8/24 (33%) of the HLA-DRB1*04 alleles in patients with reactivearthritis, accounting for most DQB1*0301 haplotypes, but onlyin 5/55 (9%) of the DRB1*04 alleles in random controls (P = 0.017).All reactive arthritis patients with this subtype were positivefor HLA-B27. DRB1*04-DQB1*0302 haplotype was increased in patientswith rheumatoid arthritis (28/92, 30%) compared with reactivearthritis (12/126, 10%) or with the controls (12/100, 12%; P =0.003). HLA-B*2705 was by far the dominant B27 subtype both inreactive arthritis patients with the particular DRB1*0408-DQB1*0301haplotype and in controls. It was found in 11 out of 12 DR analysedpatients, as well as in 10 out of 11 randomly selected B27 positivecontrols.
CONCLUSIONS $---$ Although no single class II allele was found to be increased among patients with reactive arthritis, HLA-B27, DRB1*0408, andDQB1*0301 might exert a haplotypic effect in the pathogenesisof reactive arthritis, or they may be markers of a subset of B27haplotypes conferringsusceptibility.

CiteULike

Complore

Connotea

Del.icio.us Add to Digg

Digg

Technorati What's this?

This article has been cited by other articles:

Laivoranta-Nyman, S., Möttönen, T., Luukkainen, R., Hakala, M., Yli-Kerttula, U., Hannonen, P., Tuokko, J., Toivanen, A., Ilonen, J. (2000). Immunogenetic differencies between patients with familial and non-familial rheumatoid arthritis. Ann Rheum Dis 59: 173-177 [Abstract] [Full Text]
Khare, S. D., Bull, M. J., Hanson, J., Luthra, H. S., David, C. S. (1998). Spontaneous Inflammatory Disease in HLA-B27 Transgenic Mice Is Independent of MHC Class II Molecules: A Direct Role for B27 Heavy Chains and Not B27-Derived Peptides. J. Immunol. 160: 101-106 [Abstract] [Full Text]

Services

Citing Articles

Google Scholar

PubMed

Topic Collections

Bookmark with

What's this?

Register for free content

The full back archive is now available for all BMJ Journals. Institutional subscribers may access the entire archive as part of their subscription. Personal subscribers will also have access to all content when logged in. Non-subscribers who register have free access to all articles published before 2006 right back to volume 1 issue 1. Register here to access the free archive of all BMJ Journals.

Don't forget to sign up for content alerts so you keep up to date with all the articles as they are published.