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Annals of the Rheumatic Diseases 1978;37:552-556; doi:10.1136/ard.37.6.552
Copyright © 1978 BMJ Publishing Group Ltd & European League Against Rheumatism.

The effect of zinc on alkaline phosphatase activity in rheumatoid synovial tissue.

J J Johnstone, L Bitensky, B Cashman, J Chayen

To examine the reported beneficial effect of zinc in rheumatoid arthritis, rheumatoid synovial tissue has been maintained in vitro in non-proliferative culture with or without zinc sulphate in the culture medium. Alkaline phosphatase activity was measured by microdensitometry of the cytochemical reaction in cryostat sections; the activity in blood vessels was measured separately from that in the supporting tissue below the synovial surface. Zinc enhanced this activity optimally at concentrations of between 10(-5) and 10(-4) mol/l.


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